Document Detail


Prevention by inhibitors of arachidonic acid cascade of liver carcinogenesis, cirrhosis and oxidative DNA damage caused by a choline-deficient, L-amino acid-defined diet in rats.
MedLine Citation:
PMID:  9675312     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Effects of inhibitors of arachidonic acid (AA) cascade on the development of fatty liver, cirrhosis, glutathione S-transferase placental form (GST-P)-positive preneoplastic nodules, neoplastic nodules and generation of 8-hydroxydeoxyguanosine (8-OHdG), caused by a choline-deficient, L-amino acid-defined (CDAA) diet, were examined in Fischer 344 male rats by feeding CDAA diet supplemented with the inhibitors for 12 and 30 weeks. None of the inhibitors affected fatty liver. Among cyclooxygenase (COX) inhibitors, an irreversibly acting acetylsalicylic acid and a long-acting piroxicam, and to a much lesser extent the short-acting ibuprofen but not indomethacin, inhibited the development of cirrhosis, GST-P-positive and neoplastic nodules and generation of 8-OHdG. A phospholipase A2 inhibitor p-bromophenacylbromide (BPB) also exerted similar but lesser extent of inhibitory effects. Lipoxygenase inhibitors quercetin and nordihydroguiaretic acid inhibited GST-P-positive nodules but not cirrhosis or 8-OHdG. Present results suggest that perturbed AA cascade, particularly augmented COX pathway, might play key roles in the causation of liver lesions in the CDAA diet model.
Authors:
A Denda; T Endoh; Q Tang; T Tsujiuchi; D Nakae; Y Konishi
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Mutation research     Volume:  402     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  1998 Jun 
Date Detail:
Created Date:  1998-09-01     Completed Date:  1998-09-01     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  279-88     Citation Subset:  IM    
Copyright Information:
Copyright 1998 Elsevier Science B.V. All rights reserved.
Affiliation:
Department of Oncological Pathology, Cancer Center, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634, Japan.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / administration & dosage
Animals
Arachidonic Acid / antagonists & inhibitors*
Body Weight
Choline Deficiency / complications*
Cyclooxygenase Inhibitors / pharmacology
DNA Damage*
Deoxyguanosine / analogs & derivatives,  metabolism
Feeding Behavior
Glutathione Transferase / metabolism
Lipoxygenase Inhibitors / pharmacology
Liver Cirrhosis, Experimental / pathology,  prevention & control*
Liver Neoplasms, Experimental / enzymology,  pathology,  prevention & control*
Male
Oxidative Stress*
Phospholipases A / antagonists & inhibitors
Phospholipases A2
Rats
Rats, Inbred F344
Chemical
Reg. No./Substance:
0/8-hydroxy-2'-deoxyguanosine; 0/Amino Acids; 0/Cyclooxygenase Inhibitors; 0/Lipoxygenase Inhibitors; 506-32-1/Arachidonic Acid; 961-07-9/Deoxyguanosine; EC 2.5.1.18/Glutathione Transferase; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2

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