| Prevention of apoptosis by the interaction between FIH1 and Bax. | |
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MedLine Citation:
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PMID: 21069436 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bax induces mitochondrial-dependent cell apoptosis signals in mammalian cells. However, the mechanism of how Bax is kept inactive is not fully elucidated. Here, we identify FIH1 as a potential interactor of Bax through mass spectrometry analysis. Coimmunoprecipitation and GST pull-down experiments show that FIH1 can directly interact with Bax. Bax-mediated apoptosis is suppressed by FIH1 overexpression, but accelerated by FIH1 deficiency. FIH1 functions as a cytosol retention factor of Bax, blocking Bax translocation from cytosol to mitochondria in response to apoptotic stimuli. Overall, there results unveil a novel role of FIH1 in the regulation of Bax-mediated apoptosis. |
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Authors:
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Biao Yan; Men Kong; Yi-han Chen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-11-11 |
Journal Detail:
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Title: Molecular and cellular biochemistry Volume: 348 ISSN: 1573-4919 ISO Abbreviation: Mol. Cell. Biochem. Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-01-06 Completed Date: 2011-04-18 Revised Date: 2011-08-29 |
Medline Journal Info:
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Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1-9 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Molecular Biology, College of Life Science and Technology, Tongji University, Shanghai, China. yanbiao1982@hotmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis*
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drug effects Cell Hypoxia Chromatography, High Pressure Liquid Cytosol / metabolism Hela Cells Humans Immunoprecipitation Mitochondria / metabolism Oxygen / metabolism Protein Interaction Domains and Motifs Protein Interaction Mapping Proto-Oncogene Proteins c-bcl-2 / metabolism RNA Interference Repressor Proteins / genetics, metabolism* Signal Transduction Spectrometry, Mass, Electrospray Ionization Staurosporine / pharmacology Tandem Mass Spectrometry Transfection bcl-2-Associated X Protein / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/BAX protein, human; 0/Proto-Oncogene Proteins c-bcl-2; 0/Repressor Proteins; 0/bcl-2-Associated X Protein; 62996-74-1/Staurosporine; 7782-44-7/Oxygen; EC 1.14.11.-/HIF1AN protein, human |
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