Document Detail


Prevention of LLC-PK(1) cell overgrowth in a bioartificial renal tubule device using a MEK inhibitor, U0126.
MedLine Citation:
PMID:  17884223     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A common approach to construct a bioartificial renal tubule system is to utilize renal tubular cells seeded in porous polymer membrane hollow fibers. We have reported that overgrowth of renal tubular cells was not beneficial for the transport and reabsorption functions of bioartificial tubules. Therefore, long-term maintenance of a confluent monolayer of cells in hollow fibers is essential and technically challenging. In this study, we examined whether MEK inhibitor, U0126, could maintain the monolayer of Lewis-lung cancer porcine kidney 1 (LLC-PK(1)) cells on polystyrene plates and in a dialysis module housing hollow fibers made of ethylene vinyl alcohol (EVAL). We also evaluated the leakage of urea nitrogen (UN) and creatinine (Cr) through the cell-lined hollow fibers, and reabsorption of glucose and sodium by the cells, comparing the U0126-treated cells with nontreated cells in the module. Treatment with 50micromol l(-1) U0126 prevented the overgrowth of cells cultured on polystyrene plates. Moreover, U0126-treatment reduced the leakage of UN, and increased the reabsorption of electrolytes in 65cm(2) modules. Scanning electron microscopy revealed that it also prevented the overconfluence of cells in modules. Therefore, application of U0126 is a potentially effective method to improve the performance of the device.
Authors:
Miho Inagaki; Tun A Yokoyama; Kaichiro Sawada; Vu M Duc; Genta Kanai; Jianxin Lu; Takatoshi Kakuta; Akira Saito
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-08-19
Journal Detail:
Title:  Journal of biotechnology     Volume:  132     ISSN:  0168-1656     ISO Abbreviation:  J. Biotechnol.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-10-08     Completed Date:  2007-12-19     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8411927     Medline TA:  J Biotechnol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  57-64     Citation Subset:  IM    
Affiliation:
Department of Medicine, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259-1193, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bioartificial Organs*
Biotechnology
Butadienes / pharmacology*
Cell Count
Cell Proliferation / drug effects
Enzyme Inhibitors / pharmacology*
Kidney Tubules / cytology*,  drug effects*,  metabolism
LLC-PK1 Cells
Microscopy, Electron, Scanning
Mitogen-Activated Protein Kinase Kinases / antagonists & inhibitors
Nitriles / pharmacology*
Swine
Chemical
Reg. No./Substance:
0/Butadienes; 0/Enzyme Inhibitors; 0/Nitriles; 0/U 0126; EC 2.7.12.2/Mitogen-Activated Protein Kinase Kinases

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