Document Detail


Prevention of DNA double-strand breaks induced by radioiodide-(131)I in FRTL-5 thyroid cells.
MedLine Citation:
PMID:  21190956     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Radioiodine-131 released from nuclear reactor accidents has dramatically increased the incidence of papillary thyroid cancer in exposed individuals. The deposition of ionizing radiation in cells results in double-strand DNA breaks (DSB) at fragile sites, and this early event can generate oncogenic rearrangements that eventually cause cancer. The aims of this study were to develop a method to show DNA DSBs induced by (131)I in thyroid cells; to test monovalent anions that are transported by the sodium/iodide symporter to determine whether they prevent (131)I-induced DSB; and to test other radioprotective agents for their effect on irradiated thyroid cells. Rat FRTL-5 thyroid cells were incubated with (131)I. DSBs were measured by nuclear immunofluorescence using antibodies to p53-binding protein 1 or γH2AX. Incubation with 1-10 μCi (131)I per milliliter for 90 min resulted in a dose-related increase of DSBs; the number of DSBs increased from a baseline of 4-15% before radiation to 65-90% after radiation. GH3 or CHO cells that do not transport iodide did not develop DSBs when incubated with (131)I. Incubation with 20-100 μm iodide or thiocyanate markedly attenuated DSBs. Perchlorate was about 6 times more potent than iodide or thiocyanate(.) The effects of the anions were much greater when each was added 30-120 min before the (131)I. Two natural organic compounds recently shown to provide radiation protection partially prevented DSBs caused by (131)I and had an additive effect with perchlorate. In conclusion, we developed a thyroid cell model to quantify the mitogenic effect of (131)I. (131)I causes DNA DSBs in FRTL-5 cells and had no effect on cells that do not transport iodide. Perchlorate, iodide, and thiocyanate protect against DSBs induced by (131)I.
Authors:
Jerome M Hershman; Armen Okunyan; Yelena Rivina; Sophie Cannon; Victor Hogen
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-12-29
Journal Detail:
Title:  Endocrinology     Volume:  152     ISSN:  1945-7170     ISO Abbreviation:  Endocrinology     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-02-22     Completed Date:  2011-04-27     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1130-5     Citation Subset:  AIM; IM    
Affiliation:
Endocrinology and Diabetes Division, West Los Angeles Veterans Affairs Medical Center, Los Angeles, California 90073, USA. jhershmn@ucla.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Anions / metabolism
Cell Line
DNA Breaks, Double-Stranded / drug effects*
Iodine / pharmacology*
Iodine Radioisotopes
Radiation-Protective Agents / pharmacology*
Rats
Symporters / metabolism
Thyroid Gland / cytology*
Chemical
Reg. No./Substance:
0/Anions; 0/Iodine Radioisotopes; 0/Radiation-Protective Agents; 0/Symporters; 0/sodium-iodide symporter; 7553-56-2/Iodine
Comments/Corrections

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