Document Detail


Preventing friction-induced chondrocyte apoptosis: comparison of human synovial fluid and hylan G-F 20.
MedLine Citation:
PMID:  22660808     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Symptomatic osteoarthritis (OA) is a common painful disease with limited treatment options. A rising number of patients with OA have been treated with intraarticular injections of hyaluronic acid, including the high-molecular-weight hylan G-F 20, which is injected following arthrocentesis. We investigated the effectiveness of hylan G-F 20 to lower coefficient of friction (COF) and prevent chondrocyte apoptosis in vitro.
METHODS: A disc-on-disc bovine cartilage bearing was used to measure the static and kinetic COF when lubricated with hylan G-F 20, human synovial fluid (HSF), and phosphate buffered saline (PBS). Following friction testing, we stained paraffin-embedded sections of these cartilage bearings for activated caspase-3, a marker of apoptosis.
RESULTS: Bearings lubricated with hylan G-F 20 had kinetic COF values that were similar to bearings lubricated with PBS, but significantly higher than those lubricated with HSF. There were no significant differences in static COF values in bearings lubricated with hylan G-F 20 as compared to PBS or HSF. However, bearings lubricated with HSF had significantly lower static COF values compared to bearings lubricated with PBS. The mean percentage of caspase-3-positive chondrocytes in the superficial and upper intermediate zones of bearings lubricated with hylan G-F 20 was significantly higher compared to that of bearings lubricated with HSF or unloaded controls, but significantly lower than in those lubricated with PBS.
CONCLUSION: These findings indicate that joint lubrication may prevent chondrocyte apoptosis by lowering the COF. Further, removal of synovial fluid prior to hylan G-F 20 injection may be detrimental to cartilage health.
Authors:
Kimberly A Waller; Ling X Zhang; Braden C Fleming; Gregory D Jay
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-06-01
Journal Detail:
Title:  The Journal of rheumatology     Volume:  39     ISSN:  0315-162X     ISO Abbreviation:  J. Rheumatol.     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-07-03     Completed Date:  2012-11-26     Revised Date:  2013-07-08    
Medline Journal Info:
Nlm Unique ID:  7501984     Medline TA:  J Rheumatol     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  1473-80     Citation Subset:  IM    
Affiliation:
Department of Orthopedics, and Department of Emergency Medicine, Warren Alpert Medical School of Brown University/Rhode Island Hospital, Providence, Rhode Island 02903, USA.
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MeSH Terms
Descriptor/Qualifier:
Adult
Animals
Apoptosis / drug effects*
Biocompatible Materials / pharmacology*
Caspase 3 / analysis
Cattle
Cells, Cultured
Chondrocytes / cytology,  drug effects*
Cytoprotection*
Friction*
Humans
Hyaluronic Acid / analogs & derivatives*,  pharmacology
Lubricants / pharmacology*
Male
Synovial Fluid / physiology*
Grant Support
ID/Acronym/Agency:
P20 RR024484/RR/NCRR NIH HHS; P20-RR024284/RR/NCRR NIH HHS; R01 AR050180/AR/NIAMS NIH HHS; R01-AR049199/AR/NIAMS NIH HHS; R01-AR050180/AR/NIAMS NIH HHS; R21 AR055937/AR/NIAMS NIH HHS; R21-AR055937/AR/NIAMS NIH HHS; R41 AR057276/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/Biocompatible Materials; 0/Lubricants; 125935-84-4/hylan; 9004-61-9/Hyaluronic Acid; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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