Document Detail

Prevalence of viral DNA in amniotic fluid of low-risk pregnancies in the second trimester.
MedLine Citation:
PMID:  12962262     Owner:  NLM     Status:  MEDLINE    
AIM: The association between fetal viral infection and adverse pregnancy outcome is well documented. However, the prevalence of common viral pathogens in the amniotic fluid of normal pregnancies is not established. The purpose of this study was to determine this prevalence in asymptomatic patients. METHODS: This was a prospective observational study of patients at low risk for viral infection who were referred for second-trimester genetic amniocentesis. In patients with normal fetal anatomy on ultrasound and a normal fetal karyotype, a 2-ml aliquot of amniotic fluid obtained at amniocentesis was analyzed by multiplex polymerase chain reaction for cytomegalovirus (CMV), parvovirus B19, adenovirus, enterovirus, herpes simplex virus (HSV), respiratory syncytial virus (RSV) and Epstein-Barr virus (EBV). RESULTS: Among 686 patients, advanced maternal age was the most common indication for genetic testing (n = 469, 68.4%), followed by elevated aneuploidy risk on triple screen (n = 164, 23.9%), elevated maternal serum alpha-fetoprotein (n = 20, 2.9%), previous aneuploidy (n = 16, 2.3%) and family history of inheritable disease (n = 14, 2.1%). Forty-four (6.4%) amniotic fluid samples were positive for viral genome. A single genome was amplified in 41 samples (93%). In three samples, two viral genomes were identified. Adenovirus was most frequently identified (37/44), followed by CMV (5/44), EBV (2/44), enterovirus (2/44) and RSV (1/44). Parvovirus and HSV were not identified. There was a bimodal seasonal variation in prevalence, with the highest prevalence during the summer and late winter. CONCLUSION: Viral genome is commonly found in amniotic fluid with a sonographically normal fetus, and the prevalence follows a seasonal pattern. The mechanism, significance and effects of this asymptomatic viral presence require further study.
A A Baschat; J Towbin; N E Bowles; C R Harman; C P Weiner
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians     Volume:  13     ISSN:  1476-7058     ISO Abbreviation:  J. Matern. Fetal. Neonatal. Med.     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-09-09     Completed Date:  2003-10-15     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  101136916     Medline TA:  J Matern Fetal Neonatal Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  381-4     Citation Subset:  IM    
Center for Advanced Fetal Care, Department of Obstetrics, Gynecology and Reproductive Sciences, University of Maryland, Baltimore, Maryland 21201-1703, USA.
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MeSH Terms
Adenoviridae / isolation & purification*
Amniotic Fluid / virology*
Congenital Abnormalities / genetics,  ultrasonography
Cytomegalovirus / isolation & purification*
DNA, Viral / analysis*
Enterovirus / isolation & purification*
Genetic Predisposition to Disease
Genetic Testing / methods
Herpesvirus 4, Human / isolation & purification*
Maternal Age
Polymerase Chain Reaction
Pregnancy Trimester, Second
Pregnancy, High-Risk
Prospective Studies
Respiratory Syncytial Viruses / isolation & purification*
Ultrasonography, Prenatal
alpha-Fetoproteins / analysis
Reg. No./Substance:
0/DNA, Viral; 0/alpha-Fetoproteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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