| Prevalence of plasmid-mediated AmpC beta-lactamases among Enterobacteriaceae in Algiers hospitals. | |
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MedLine Citation:
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PMID: 19570655 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study was to investigate the prevalence and diversity of plasmid-mediated AmpC cephalosporinases (PAcBLs) in clinical isolates of Enterobacteriaceae collected between 2003 and 2007 from three Algiers hospitals. Antibiograms were determined on Mueller-Hinton agar plates using the disk diffusion method, and minimum inhibitory concentrations were determined by Etest. Isolates resistant to cefoxitin or ceftazidime were screened for bla(CMY), bla(DHA), bla(FOX) and bla(ACC) as well as extended-spectrum beta-lactamase (ESBL) genes by polymerase chain reaction (PCR). PCR products were sequenced by the Sanger method. Plasmid incompatibility grouping was conducted by PCR-based replicon typing. The prevalence of PAcBLs was 2.18% (11/505), comprising 8 CMY-2 and 3 DHA-1 enzymes. CTX-M-15 was co-produced with CMY-2 in three isolates and with DHA-1 in one isolate; the two remaining DHA-1-producers co-expressed SHV-12 ESBL. This is the first report of plasmid-mediated AmpC from Algeria, with the first detection of DHA-1 in Enterobacter cloacae. |
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Authors:
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Hassen Iabadene; Yamina Messai; Houria Ammari; Souhila Alouache; Charlotte Verdet; Rabah Bakour; Guillaume Arlet |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-07-01 |
Journal Detail:
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Title: International journal of antimicrobial agents Volume: 34 ISSN: 1872-7913 ISO Abbreviation: Int. J. Antimicrob. Agents Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-08-03 Completed Date: 2009-10-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9111860 Medline TA: Int J Antimicrob Agents Country: Netherlands |
Other Details:
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Languages: eng Pagination: 340-2 Citation Subset: IM |
Affiliation:
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Laboratoire de Biologie Cellulaire et Moléculaire, Faculté des Sciences Biologiques, Université des Sciences et de la Technologie Houari Boumediene, BP 32 El-Alia, Bab-Ezzouar 16111, Alger, Algeria. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Algeria
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epidemiology Anti-Bacterial Agents / pharmacology Bacterial Proteins / genetics*, metabolism Cefoxitin / pharmacology Ceftazidime / pharmacology Cross Infection / epidemiology*, microbiology Enterobacteriaceae / drug effects*, enzymology, genetics Enterobacteriaceae Infections / epidemiology*, microbiology Hospitals Humans Microbial Sensitivity Tests Plasmids / genetics* Polymerase Chain Reaction Prevalence beta-Lactam Resistance beta-Lactamases / genetics*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/Bacterial Proteins; 35607-66-0/Cefoxitin; 78439-06-2/Ceftazidime; EC 3.5.2.6/AmpC beta-lactamases; EC 3.5.2.6/beta-Lactamases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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