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Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in dairy cattle in Ningxia, northwestern China.
MedLine Citation:
PMID:  25488627     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Background Cryptosporidium spp. and Giardia duodenalis are important gastrointestinal protists in humans and animals worldwide. In China, bovine cryptosporidiosis and giardiasis are of increasing concern because cattle are important reservoirs of these parasites, which have become potential threats to public health and to large numbers of cattle in recent years.ResultsA total of 1366 fecal samples from the Ningxia Autonomous Region were examined. The overall infection rates for Cryptosporidium spp. and G. duodenalis were 1.61% and 2.12%, respectively. Cryptosporidium was only detected in preweaned calves and adults older than 2 years, whereas G. duodenalis was only detected in calves aged less than 11 months. Cryptosporidium spp. were characterized with a PCR¿restriction fragment length polymorphism analysis and DNA sequence analysis of the small subunit rRNA gene. Three Cryptosporidium species were identified: C. parvum (n =15) and C. bovis (n =4) in preweaned calves, and C. andersoni (n =4) in adults aged over 2 years. A DNA sequence analysis of the gp60 gene suggested that the 15 C. parvum isolates all belonged to subtype IIdA15G1. Twenty-nine G. duodenalis isolates were analyzed by DNA sequencing of the triosephosphate isomerase (tpi) and glutamate dehydrogenase (gdh) genes. Two G. duodenalis assemblages were identified, assemblages E (n =15) and B (n =4, one subtype B1 and three subtype B2) in preweaned calves, and assemblage E (n =10) in 3¿11-month-old calves.ConclusionsThe predominance of C. parvum detected in preweaned calves and the first identified subtype IIdA15G1 in dairy cattle, and the dominant G. duodenalis assemblage E in this study differed considerably from those found in Henan, Heilongjiang, and Shannxi Provinces. Our findings further confirm the dominance of C. parvum IId subtypes in China.
Authors:
Jianying Huang; Daoyou Yue; Meng Qi; Rongjun Wang; Jinfeng Zhao; Junqiang Li; Ke Shi; Ming Wang; Longxian Zhang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-12-9
Journal Detail:
Title:  BMC veterinary research     Volume:  10     ISSN:  1746-6148     ISO Abbreviation:  BMC Vet. Res.     Publication Date:  2014 Dec 
Date Detail:
Created Date:  2014-12-9     Completed Date:  -     Revised Date:  2014-12-10    
Medline Journal Info:
Nlm Unique ID:  101249759     Medline TA:  BMC Vet Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  292     Citation Subset:  -    
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