Document Detail


Prevalence of influenza virus among the paediatric population in Mumbai during 2007-2009.
MedLine Citation:
PMID:  22664429     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Influenza has a major impact on public heath, annually affecting 15-20% of the global population. Information on the activity of influenza virus in Mumbai is limited. The present study was carried out to determine the prevalence of influenza viruses causing acute respiratory infections in children by molecular methods.
OBJECTIVE: To study the prevalence of influenza viruses among the paediatric population in Mumbai by real-time reverse-transcriptase polymerase chain reaction (rRT-PCR).
MATERIALS AND METHODS: From July 2007 to July 2009, 100 respiratory samples (nasal and throat swabs) were collected from paediatric patients with acute respiratory symptoms. attending out patients department, and admitted to the paediatric wards of B. J. Wadia Hospital for Children, Mumbai. The samples were collected and processed as per World Health Organization (WHO) guidelines. Viral RNA was extracted and one-step rRT-PCR was performed to detect influenza type A (H1 and H3) and influenza type B virus.
RESULTS: Out of 100 samples processed by rRT-PCR, a total of 11 samples (11%) were positive for influenza virus. The typing for influenza A subtypes showed 1% (1) positivity for H1 and 5% (5) positivity for H3 subtypes and 5% (5) samples tested positive for influenza type B virus.
CONCLUSION: It was observed that both influenza type A and B viruses were prevalent in Mumbai during the study period. Such surveillance data are important in the early detection of any antigenic variants that may be helpful in global influenza vaccine preparation and for any pandemic preparedness activity.
Authors:
S Roy; D Patil; R Dahake; S Mukherjee; S V Athlekar; R A Deshmukh; A Chowdhary
Related Documents :
22579629 - Detection and typing of viruses using broadly sensitive cocktail-pcr and mass spectrome...
22374699 - Tissue factor and glycoprotein c on herpes simplex virus type 1 are protease activated ...
17972749 - Transmission dynamics of the amphibian ranavirus ambystoma tigrinum virus.
22787269 - Complete genome sequence of bluetongue virus serotype 16 of goat origin from india.
22720709 - In vitro propagation of chicken anemia virus in chicken peripheral blood mononuclear ce...
22372249 - Detection of viral hemorrhagic septicemia virus by quantitative reverse transcription p...
6602089 - Stimulation of cytotoxic t cells by liposomes containing influenza virus or its compone...
11750139 - Sensitivity of primary cells immortalised by oncogene transfection for the detection an...
4055489 - An epizootic of respiratory syncytial virus infection in a dairy herd.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Indian journal of medical microbiology     Volume:  30     ISSN:  1998-3646     ISO Abbreviation:  Indian J Med Microbiol     Publication Date:    2012 Apr-Jun
Date Detail:
Created Date:  2012-06-05     Completed Date:  2012-09-28     Revised Date:  2014-07-31    
Medline Journal Info:
Nlm Unique ID:  8700903     Medline TA:  Indian J Med Microbiol     Country:  India    
Other Details:
Languages:  eng     Pagination:  155-8     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Child
Child, Preschool
Female
Humans
India / epidemiology
Infant
Influenza A virus / genetics,  isolation & purification*
Influenza B virus / genetics,  isolation & purification*
Influenza, Human / diagnosis*,  epidemiology*,  virology
Male
Molecular Diagnostic Techniques / methods
Nasal Mucosa / virology
Pharynx / virology
Prevalence
RNA, Viral / genetics,  isolation & purification
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Virology / methods
Chemical
Reg. No./Substance:
0/RNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Serum hepatitis B surface antigen levels correlate with high serum HBV DNA levels in patients with c...
Next Document:  The non-association of Panton-Valentine leukocidin and mecA genes in the genome of Staphylococcus au...