Document Detail


Prevalence of alpha-chain variants at birth.
MedLine Citation:
PMID:  7457429     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hemoglobin electrophoresis of cord blood from 4,499 newborns was performed as part of a sickle cell disease detection program. Although the expected frequency of the genes for hemoglobins S and C were observed, six newborns (five kindreds) were heterozygous for an alpha G Hb. In four kindreds, the alpha chain variant was identified as Hb G-Philadelphia. In each case, heterozygosity for this Hb was manifested at birth by the presence of Hb F/G (alpha 2 G gamma 2), a slow Hb migrating between the positions of Hbs S and C on cellulose acetate. In some newborns, Hb G (alpha 2 G beta 2) was also detectable, so that these cord bloods had four hemoglobin components: F, F/G, A, and G. The prevalence of Hb G-Philadelphia in this population sample is higher than that usually reported for black Americans and may represent a founder effect. Comprehensive screening of cord blood hemoglobins should use electrophoresis on alkaline media as the primary testing procedure because it allows recognition of most variants, such as the D (G) hemoglobins. Reliable identification of Hbs S and C requires citrate agar electrophoresis as a confirmatory test.
Authors:
O Castro; W P Winter; T C Lee; V E Headings
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of clinical pathology     Volume:  75     ISSN:  0002-9173     ISO Abbreviation:  Am. J. Clin. Pathol.     Publication Date:  1981 Jan 
Date Detail:
Created Date:  1981-03-27     Completed Date:  1981-03-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370470     Medline TA:  Am J Clin Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  56-9     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Blood Protein Electrophoresis
Fetal Blood / analysis*
Hemoglobin, Sickle / analysis
Hemoglobins / genetics
Hemoglobins, Abnormal / analysis*
Heterozygote
Humans
Infant, Newborn
Grant Support
ID/Acronym/Agency:
15160//PHS HHS
Chemical
Reg. No./Substance:
0/Hemoglobin, Sickle; 0/Hemoglobins; 0/Hemoglobins, Abnormal

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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