| Prevalence of Staphylococcus aureus strains in an Australian cohort, 1989-2003: evidence for the low prevalence of the toxic shock toxin and Panton-Valentine leukocidin genes. | |
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MedLine Citation:
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PMID: 19466470 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The purpose of this paper is to determine the prevalence of the toxic shock toxin gene (tst) and to enumerate the circulating strains of methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant S. aureus (MRSA) in Australian isolates collected over two decades. The aim was to subtype these strains using the binary genes pvl, cna, sdrE, pUB110 and pT181. Isolates were assayed using real-time polymerase chain reaction (PCR) for mecA, nuc, 16 S rRNA, eight single-nucleotide polymorphisms (SNPs) and for five binary genes. Two real-time PCR assays were developed for tst. The 90 MRSA isolates belonged to CC239 (39 in 1989, 38 in 1996 and ten in 2003), CC1 (two in 2003) and CC22 (one in 2003). The majority of the 210 MSSA isolates belonged to CC1 (26), CC5 (24) and CC78 (23). Only 18 isolates were tst-positive and only 15 were pvl-positive. Nine MSSA isolates belonged to five binary types of ST93, including two pvl-positive types. The proportion of tst-positive and pvl-positive isolates was low and no significant increase was demonstrated. Dominant MSSA clonal complexes were similar to those seen elsewhere, with the exception of CC78. CC239 MRSA (AUS-2/3) was the predominant MRSA but decreased significantly in prevalence, while CC22 (EMRSA-15) and CC1 (WA-1) emerged. Genetically diverse ST93 MSSA predated the emergence of ST93-MRSA (the Queensland clone). |
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Authors:
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S Schlebusch; J M Schooneveldt; F Huygens; G R Nimmo |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-05-24 |
Journal Detail:
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Title: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology Volume: 28 ISSN: 1435-4373 ISO Abbreviation: Eur. J. Clin. Microbiol. Infect. Dis. Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-10-02 Completed Date: 2009-11-09 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8804297 Medline TA: Eur J Clin Microbiol Infect Dis Country: Germany |
Other Details:
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Languages: eng Pagination: 1183-9 Citation Subset: IM |
Affiliation:
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Department of Microbiology, Pathology Queensland Central Laboratory, Herston Hospital Complex, Herston, 4029, QLD, Australia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Australia Bacterial Toxins / genetics* Cohort Studies DNA, Bacterial / chemistry, genetics Enterotoxins / genetics* Exotoxins / genetics* Genotype Humans Leukocidins / genetics* Methicillin-Resistant Staphylococcus aureus / classification, drug effects, genetics Molecular Epidemiology Polymerase Chain Reaction Polymorphism, Single Nucleotide Prevalence Staphylococcal Infections / drug therapy, epidemiology*, microbiology* Staphylococcus aureus / classification, drug effects, genetics* Superantigens / genetics* Virulence Factors / genetics |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Toxins; 0/DNA, Bacterial; 0/Enterotoxins; 0/Exotoxins; 0/Leukocidins; 0/Panton-Valentine leukocidin; 0/Superantigens; 0/Virulence Factors; 0/enterotoxin F, Staphylococcal |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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