| Prevalence of Fc-gammaR chain expression in CD4+ T cells of patients with systemic lupus erythematosus. | |
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MedLine Citation:
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PMID: 16134728 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To determine the upregulation of transcript and protein levels of the T cell receptor (TCR)/CD3-Fc-gammaR chain in CD4+ T cells of systemic lupus erythematosus (SLE) patients with different clinical disease activity scored on the SLE Disease Activity Index (SLEDAI) scale. METHODS: CD4+ cells were isolated by the positive biomagnetic separation technique. Quantitative analysis of Fc-gammaR cDNA was carried out by using the real-time quantitative polymerase chain reaction (RQ-PCR) SYBR Green I system. Protein levels of Fc-gammaR in CD4+ T cells were determined by Western blotting analysis. RESULTS: We observed significantly higher transcript and protein levels of the Fc-gammaR chain in CD4+ T cells of SLE patients (n = 45) than in healthy individuals (n = 26). The increase in Fc-gammaR expression was observed in 97.8% of SLE patients. Spearman statistical analysis suggests that the protein level of Fc-gammaR in CD4+ T cells may correlate with SLE activity scored by the SLEDAI scale (R = 0.556, p < 0.00006, respectively). CONCLUSION: The high prevalence of the Fc-gammaR chain in CD4+ T cells of SLE patients may indicate an important role for this molecule in the pathogenesis of SLE. |
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Authors:
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L Butkiewicz; S Duriagin; R Laddach; H Chwalinska-Sadowska; P P Jagodzinski |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Scandinavian journal of rheumatology Volume: 34 ISSN: 0300-9742 ISO Abbreviation: Scand. J. Rheumatol. Publication Date: 2005 May-Jun |
Date Detail:
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Created Date: 2005-09-01 Completed Date: 2005-09-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0321213 Medline TA: Scand J Rheumatol Country: Norway |
Other Details:
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Languages: eng Pagination: 216-9 Citation Subset: IM |
Affiliation:
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Institute of Reumatology, Warsaw, Poland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged CD4-Positive T-Lymphocytes / immunology*, metabolism* Female Gene Expression Regulation Health Status Humans Lupus Erythematosus, Systemic / immunology*, metabolism*, physiopathology Male Middle Aged RNA, Messenger / metabolism Receptor-CD3 Complex, Antigen, T-Cell / genetics, immunology*, metabolism* Reverse Transcriptase Polymerase Chain Reaction Severity of Illness Index Up-Regulation |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 0/Receptor-CD3 Complex, Antigen, T-Cell |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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