Document Detail


Prevalence of Fc-gammaR chain expression in CD4+ T cells of patients with systemic lupus erythematosus.
MedLine Citation:
PMID:  16134728     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To determine the upregulation of transcript and protein levels of the T cell receptor (TCR)/CD3-Fc-gammaR chain in CD4+ T cells of systemic lupus erythematosus (SLE) patients with different clinical disease activity scored on the SLE Disease Activity Index (SLEDAI) scale. METHODS: CD4+ cells were isolated by the positive biomagnetic separation technique. Quantitative analysis of Fc-gammaR cDNA was carried out by using the real-time quantitative polymerase chain reaction (RQ-PCR) SYBR Green I system. Protein levels of Fc-gammaR in CD4+ T cells were determined by Western blotting analysis. RESULTS: We observed significantly higher transcript and protein levels of the Fc-gammaR chain in CD4+ T cells of SLE patients (n = 45) than in healthy individuals (n = 26). The increase in Fc-gammaR expression was observed in 97.8% of SLE patients. Spearman statistical analysis suggests that the protein level of Fc-gammaR in CD4+ T cells may correlate with SLE activity scored by the SLEDAI scale (R = 0.556, p < 0.00006, respectively). CONCLUSION: The high prevalence of the Fc-gammaR chain in CD4+ T cells of SLE patients may indicate an important role for this molecule in the pathogenesis of SLE.
Authors:
L Butkiewicz; S Duriagin; R Laddach; H Chwalinska-Sadowska; P P Jagodzinski
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Scandinavian journal of rheumatology     Volume:  34     ISSN:  0300-9742     ISO Abbreviation:  Scand. J. Rheumatol.     Publication Date:    2005 May-Jun
Date Detail:
Created Date:  2005-09-01     Completed Date:  2005-09-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0321213     Medline TA:  Scand J Rheumatol     Country:  Norway    
Other Details:
Languages:  eng     Pagination:  216-9     Citation Subset:  IM    
Affiliation:
Institute of Reumatology, Warsaw, Poland.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
CD4-Positive T-Lymphocytes / immunology*,  metabolism*
Female
Gene Expression Regulation
Health Status
Humans
Lupus Erythematosus, Systemic / immunology*,  metabolism*,  physiopathology
Male
Middle Aged
RNA, Messenger / metabolism
Receptor-CD3 Complex, Antigen, T-Cell / genetics,  immunology*,  metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Severity of Illness Index
Up-Regulation
Chemical
Reg. No./Substance:
0/RNA, Messenger; 0/Receptor-CD3 Complex, Antigen, T-Cell

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