Document Detail

Prevalence of Chlamydiaceae and Mollicutes on the genital mucosa and serological findings in dairy cattle.
MedLine Citation:
PMID:  17890023     Owner:  NLM     Status:  MEDLINE    
It was the aim of this project to obtain information on the prevalence of Chlamydiaceae and Mollicutes and their potential importance for reproductive problems in cattle. Cervical or vaginal swabs were taken from 644 animals in 196 farms and blood samples were collected from 375 cattle. Out of the animals, 6.8% had aborted within the last 12 months, 2.6% showed clinical vaginitis and 11.6% clinical endometritis. Chlamydiaceae were detected and identified by PCR followed by restriction fragment length polymorphism (RFLP) analysis. For the detection and identification of Mollicutes cultivation procedures, biochemical differentiation and serological identification were used. Sera were tested for antibodies against Chlamydiaceae and Mycoplasma (M.) bovis by ELISA and against M. bovigenitalium by Western blot analysis. Chlamydophila (Cp.) abortus was found in three cervical swabs. Cp. pecorum was detected in 9% of cervical or vaginal swabs. The majority of Cp. species found was Cp. pecorum and thus fertility problems caused by Cp. abortus are limited. M. bovis was found in only one genital swab. M. bovigenitalium was rarely diagnosed (3% of cervical and 2% of vaginal swabs). M. bovigenitalium was found more often in cattle having aborted (4/32 animals) than in cattle without history of abortion (5/220, p<0.05). Ureaplasma (U.) diversum existed in 12% of cervical and 36% of vaginal swabs and was found in 8 out of 17 animals with vaginitis. Out of the animals tested, 44.9% were seropositive for Chlamydiaceae, 14.8% for M. bovis and 27.3% for M. bovigenitalium.
Tom Petit; Joachim Spergser; Jörg Aurich; Renate Rosengarten
Related Documents :
24577433 - Impact of race on male predisposition to birth asphyxia.
12461493 - Alcohol dehydrogenase 2 genotypes, maternal alcohol use, and infant outcome.
11453263 - Operative vaginal delivery: clinical appraisal of a new vacuum extraction device.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-08-19
Journal Detail:
Title:  Veterinary microbiology     Volume:  127     ISSN:  0378-1135     ISO Abbreviation:  Vet. Microbiol.     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-02-04     Completed Date:  2008-05-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705469     Medline TA:  Vet Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  325-33     Citation Subset:  IM    
Clinic for Obstetrics, Gynaecology and Andrology, University of Veterinary Sciences, 1210 Vienna, Austria.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Abortion, Veterinary / microbiology
Antibodies, Bacterial / blood
Austria / epidemiology
Blotting, Western / methods,  veterinary
Cattle Diseases / diagnosis,  epidemiology*,  microbiology
Cervix Uteri / microbiology
Chlamydiaceae / isolation & purification*
Chlamydiaceae Infections / diagnosis,  epidemiology,  veterinary*
Enzyme-Linked Immunosorbent Assay / methods,  veterinary
Genital Diseases, Female / diagnosis,  epidemiology,  microbiology,  veterinary*
Gram-Negative Bacterial Infections / diagnosis,  epidemiology,  veterinary*
Mollicutes / isolation & purification*
Mucous Membrane / microbiology
Polymerase Chain Reaction / methods,  veterinary
Polymorphism, Restriction Fragment Length
Pregnancy Complications, Infectious / diagnosis,  epidemiology,  veterinary
Vagina / microbiology
Reg. No./Substance:
0/Antibodies, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Fla-DGGE analysis of Campylobacter jejuni and Campylobacter coli in cecal samples of broilers withou...
Next Document:  BLT-1, a specific inhibitor of the HDL receptor SR-BI, induces a copper-dependent phenotype during z...