| Prevalence of Bacillus anthracis-like organisms and bacteriophages in the intestinal tract of the earthworm Eisenia fetida. | |
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MedLine Citation:
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PMID: 20118353 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Stable infection of Bacillus anthracis laboratory strains with environmental bacteriophages confers survival phenotypes in soil and earthworm intestinal niches (R. Schuch and V. A. Fischetti, PLoS One 4:e6532, 2009). Here, the natural occurrence of two such B. anthracis-infective bacteriophages, Wip1 and Wip4, was examined in the intestines of Eisenia fetida earthworms as part of a 6-year longitudinal study at a Pennsylvania forest site. The Wip1 tectivirus was initially dominant before being supplanted by the Wip4 siphovirus, which was then dominant for the next 3 years. In a host range analysis of a wide-ranging group of Bacillus species and related organisms, Wip1 and Wip4 were both infective only toward B. anthracis and certain B. cereus strains. The natural host of Wip4 remained constant for 3 years and was a B. cereus strain that expressed a B. anthracis-like surface polysaccharide at septal positions on the cell surface. Next, a novel metagenomic approach was used to determine the extent to which such B. cereus- and B. anthracis-like strains are found in worms from two geographical locations. Three different enrichment strategies were used for metagenomic DNA isolation, based either on the ability of B. cereus sensu lato to form heat-resistant spores, the sensitivity of B. anthracis to the PlyG lysin, or the selective amplification of environmental phages cocultured with B. anthracis. Findings from this work indicate that B. cereus sensu lato and its phages are common inhabitants of earthworm intestines. |
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Authors:
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R Schuch; A J Pelzek; S Kan; V A Fischetti |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-01-29 |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 76 ISSN: 1098-5336 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-23 Completed Date: 2010-06-07 Revised Date: 2010-10-04 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 2286-94 Citation Subset: IM |
Affiliation:
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Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, 1230 York Ave., New York, NY 10065, USA. schuchr@rockefeller.edu |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/GU142940; GU142941; GU142942; GU142943; GU142944; GU142945; GU142946; GU142947; GU142948 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bacillus Phages / classification, genetics, growth & development, isolation & purification* Bacillus anthracis / classification, genetics, isolation & purification*, virology* Bacillus cereus / virology Cluster Analysis DNA, Bacterial / chemistry, genetics DNA, Ribosomal / chemistry, genetics DNA, Viral / chemistry, genetics Gastrointestinal Tract / microbiology, virology Metagenome Molecular Sequence Data Oligochaeta / microbiology*, virology* Pennsylvania Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Viral Proteins / genetics |
| Grant Support | |
ID/Acronym/Agency:
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AI057472/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 0/DNA, Ribosomal; 0/DNA, Viral; 0/RNA, Ribosomal, 16S; 0/Viral Proteins |
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