Document Detail


Pretreatment with perfluorohexane vapor attenuates fMLP-induced lung injury in isolated perfused rabbit lungs.
MedLine Citation:
PMID:  20653469     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The authors investigated the protective effects and dose dependency of perfluorohexane (PFH) vapor on leukocyte-mediated lung injury in isolated, perfused, and ventilated rabbit lungs. Lungs received either 18 vol.% (n = 7), 9 vol.% (n = 7), or 4.5 vol.% (n = 7) PFH. Fifteen minutes after beginning of PFH application, lung injury was induced with formyl-Met-Leu-Phe (fMLP). Control lungs (n = 7) received fMLP only. In addition 5 lungs (PFH-sham) remained uninjured receiving 18 vol.% PFH only. Pulmonary artery pressure (mPAP), peak inspiratory pressure (P(max)), and lung weight were monitored for 90 minutes. Perfusate samples were taken at regular intervals for analysis and representative lungs were fixed for histological analysis. In the control, fMLP application led to a significant increase of mPAP, P(max), lung weight, and lipid mediators. Pretreatment with PFH attenuated the rise in these parameters. This was accompanied by preservation of the structural integrity of the alveolar architecture and air-blood barrier. In uninjured lungs, mPAP, P(max), lung weight, and lipid mediator formation remained uneffected in the presence of PFH. The authors concluded that pretreatment with PFH vapor leads to an attenuation of leukocyte-mediated lung injury. Vaporization of perfluorocarbons (PFCs) offers new therapeutic options, making use of their protective and anti-inflammatory properties in prophylaxis or in early treatment of acute lung injury.
Authors:
Jörg U Bleyl; Axel R Heller; Antonia Fehrenbach; Manuel Heintz; Heinz Fehrenbach; Gesa Klenz; Marcelo Gama de Abreu; Matthias Hübler; Peter M Spieth; Thea Koch
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental lung research     Volume:  36     ISSN:  1521-0499     ISO Abbreviation:  Exp. Lung Res.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-26     Completed Date:  2010-11-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8004944     Medline TA:  Exp Lung Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  342-51     Citation Subset:  IM    
Affiliation:
Clinic for Anaesthesiology and Intensive Care Therapy, University Hospital Carl Gustav Carus, Technical University of Dresden, Dresden, Germany. joerg.bleyl@uniklinikum-dresden.de
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MeSH Terms
Descriptor/Qualifier:
Animals
Anti-Inflammatory Agents / pharmacology*
Blood Pressure / drug effects
Cytoprotection
Disease Models, Animal
Dose-Response Relationship, Drug
Fluorocarbons / pharmacology*
Inflammation Mediators / metabolism
Inhalation / drug effects
Leukocytes / drug effects,  immunology
Lung / blood supply,  drug effects*,  immunology,  pathology,  physiopathology
Lung Injury / chemically induced,  immunology,  pathology,  physiopathology,  prevention & control*
N-Formylmethionine Leucyl-Phenylalanine*
Organ Size / drug effects
Perfusion
Pulmonary Artery / drug effects,  physiopathology
Pulmonary Edema / chemically induced,  prevention & control
Rabbits
Time Factors
Volatilization
Chemical
Reg. No./Substance:
0/Anti-Inflammatory Agents; 0/Fluorocarbons; 0/Inflammation Mediators; 355-42-0/perfluorohexane; 59880-97-6/N-Formylmethionine Leucyl-Phenylalanine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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