Document Detail


Pretreatment with glutamine attenuates anoxia/reoxygenation injury of human proximal renal tubular epithelial cells via induction of heme oxygenase-1.
MedLine Citation:
PMID:  19468257     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND/AIMS: Glutamine showed potential cytoprotective activity probably due to induction of heme oxygenase-1 (HO-1). We thus investigated the effect of glutamine in vitro on anoxia/reoxygenation (A/R) injury of renal tubular epithelial cells. METHODS: Human proximal renal tubular epithelial cells (HK-2) were subjected to A/R with or without glutamine, after which viability and apoptosis of the cells were determined by MTT assay and annexin V flow cytometry, respectively. Meanwhile, expression of HO-1, caspase-3, Bcl-2 and inducible nitric oxide synthetase (iNOS) was measured by reverse transcriptase polymerase chain reaction and Western blot. RESULTS: A/R significantly induced apoptosis of HK-2 cells, which was inhibited by glutamine, during which HO-1 was upregulated significantly, but nullified by p38MAPK inhibitor. Caspase-3 and iNOS increased after A/R; however, glutamine inhibited this potential. Opposite results were observed for Bcl-2. CONCLUSION: Glutamine prevents A/R injury of HK-2 cells through induction of HO-1 via a p38MAPK-dependent pathway, which plays a key role in the cytoprotective effect of glutamine.
Authors:
Qiang Shi; Ya-Ni Feng; Jun Fang; Ke Xu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-05-20
Journal Detail:
Title:  Pharmacology     Volume:  84     ISSN:  1423-0313     ISO Abbreviation:  Pharmacology     Publication Date:  2009  
Date Detail:
Created Date:  2009-07-17     Completed Date:  2009-12-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0152016     Medline TA:  Pharmacology     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  1-8     Citation Subset:  IM    
Affiliation:
Department of Radiology, The First Affiliated Hospital, China Medical University, Shenyang, China.
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MeSH Terms
Descriptor/Qualifier:
Antioxidants / pharmacology*
Apoptosis / drug effects
Blotting, Western
Caspase 3 / metabolism
Cell Hypoxia
Cells, Cultured
Cytoprotection
Epithelial Cells / drug effects*,  enzymology,  pathology
Glutamine / pharmacology*
Heme Oxygenase-1 / antagonists & inhibitors,  biosynthesis*
Humans
Kidney Tubules, Proximal / drug effects*,  enzymology,  pathology
Nitric Oxide / genetics
Nitric Oxide Synthase / metabolism
Oxidative Stress / drug effects
Oxygen / pharmacology*
Proto-Oncogene Proteins c-bcl-2 / metabolism
Reperfusion Injury / enzymology,  pathology,  prevention & control
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Proto-Oncogene Proteins c-bcl-2; 10102-43-9/Nitric Oxide; 56-85-9/Glutamine; 7782-44-7/Oxygen; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.14.99.3/Heme Oxygenase-1; EC 3.4.22.-/Caspase 3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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