| Pretreatment with arginine preserves intestinal barrier integrity and reduces bacterial translocation in mice. | |
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MedLine Citation:
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PMID: 19660909 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To evaluate the effects of arginine on intestinal barrier integrity and bacterial translocation (BT) in mice undergoing intestinal obstruction. METHODS: Mice were divided into 3 groups, treated for 7 d before surgical intervention with isocaloric and isoprotein diets. The ARG group received a diet containing 2% arginine, the IO (intestinal obstruction) and Sham groups, standard chow diet. On the eighth day of treatment, all animals received diethylenetriamine pentaacetic acid (DTPA) solution labeled with 99mTechnetium (99mTc-DTPA) by gavage for intestinal permeability analysis. After 90 min, the animals were anesthetized and the terminal ileum ligated. The Sham group only underwent laparotomy. After 4, 8, and 18 h, blood was collected for radioactivity determination. Samples of ileum were collected 18 h after surgery for histological analysis. In another set of animals, BT was evaluated. After 7 d of treatment, all animals received 10(8) CFU/mL of 99mTc-E.coli by gavage; 90 min later they were submitted to the surgical procedure described above. BT was determined by the uptake of 99mTc-E.coli in blood, mesenteric lymph nodes, liver, spleen, and lungs, assessed 18 h after the surgery. RESULTS: The intestinal permeability and BT were higher in the IO group when compared with the Sham group (P < 0.05). Arginine supplementation reduced intestinal permeability and BT to physiologic levels. Histological analysis showed mucosal ileum preservation in animals treated with arginine. CONCLUSION: Arginine was able to preserve barrier integrity, thus reducing BT. |
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Authors:
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Mirelle L Viana; Rosana G C Santos; Simone V Generoso; Rosa M E Arantes; Maria Isabel T D Correia; Valbert N Cardoso |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-08-05 |
Journal Detail:
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Title: Nutrition (Burbank, Los Angeles County, Calif.) Volume: 26 ISSN: 1873-1244 ISO Abbreviation: Nutrition Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-02-03 Completed Date: 2010-05-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8802712 Medline TA: Nutrition Country: United States |
Other Details:
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Languages: eng Pagination: 218-23 Citation Subset: IM |
Copyright Information:
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2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Programa de P?s Gradua??o em Ci?ncia de Alimentos, Faculdade de Farm?cia; Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil. mirellenut@yahoo.com.br |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arginine / administration & dosage, pharmacology* Bacterial Translocation / drug effects* Blood / drug effects, microbiology Diet Escherichia coli / physiology* Escherichia coli Infections / prevention & control Ileum / drug effects, microbiology, pathology Intestinal Mucosa / drug effects*, microbiology, pathology Intestinal Obstruction* Isotopes Liver / drug effects, microbiology Lung / drug effects, microbiology Lymph Nodes / drug effects, microbiology Mice Pentetic Acid Permeability Spleen / drug effects, microbiology Technetium / diagnostic use |
| Chemical | |
Reg. No./Substance:
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0/Isotopes; 67-43-6/Pentetic Acid; 74-79-3/Arginine; 7440-26-8/Technetium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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