Document Detail

Pretreatment of eucalyptus wood chips for enzymatic saccharification using combined sulfuric acid-free ethanol cooking and ball milling.
MedLine Citation:
PMID:  17546689     Owner:  NLM     Status:  MEDLINE    
A combined sulfuric acid-free ethanol cooking and pulverization process was developed in order to achieve the complete saccharification of the cellulosic component of woody biomass, thereby avoiding the problems associated with the use of strong acid catalysts. Eucalyptus wood chips were used as a raw material and exposed to an ethanol/water/acetic acid mixed solvent in an autoclave. This process can cause the fibrillation of wood chips. During the process, the production of furfural due to an excessive degradation of polysaccharide components was extremely low and delignification was insignificant. Therefore, the cooking process is regarded not as a delignification but as an activation of the original wood. Subsequently, the activated solid products were pulverized by ball-milling in order to improve their enzymatic digestibility. Enzymatic hydrolysis experiments demonstrated that the conversion of the cellulosic components into glucose attained 100% under optimal conditions. Wide-angle X-ray diffractometry and particle size distribution analysis revealed that the scale affecting the improvement of enzymatic digestibility ranged from 10 nm to 1 microm. Field emission scanning electron microscopy depicted that the sulfuric acid-free ethanol cooking induced a pore formation by the removal of part of the lignin and hemicellulose fractions in the size range from a few of tens nanometers to several hundred nanometers.
Yoshikuni Teramoto; Noriko Tanaka; Seung-Hwan Lee; Takashi Endo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  99     ISSN:  1097-0290     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2007-12-06     Completed Date:  2008-01-23     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  75-85     Citation Subset:  IM    
Copyright Information:
(c) 2007 Wiley Periodicals, Inc.
National Institute of Advanced Industrial Science and Technology (AIST), Biomass Technology Research Center (BTRC), 2-2-2, Hirosuehiro, Kure, Hiroshima 737-0197, Japan.
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MeSH Terms
Cellulase / chemistry*
Enzyme Activation
Ethanol / chemistry*
Eucalyptus / chemistry*
Hot Temperature
Polysaccharides / chemistry*
Sulfuric Acids / chemistry
Wood / chemistry*
Reg. No./Substance:
0/Polysaccharides; 0/Sulfuric Acids; 64-17-5/Ethanol; 7664-93-9/sulfuric acid; 8024-50-8/hemicellulose; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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