Document Detail


Pretreatment but not treatment with probiotics abolishes mouse intestinal barrier dysfunction in acute pancreatitis.
MedLine Citation:
PMID:  19167970     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Intestinal barrier failure during acute pancreatitis (AP) is associated with translocation of luminal bacteria, resulting in infectious complications. We examined the effects of multispecies probiotics on the intestinal barrier impairment in a murine model of AP. METHODS: Mice were injected with cerulein to induce AP and were sacrificed 11 (early AP) or 72 hours (late AP) after start of induction. AP and associated systemic effects were confirmed by histology of pancreas and lung. Animals received daily probiotics starting 2 days prior to AP induction (pretreatment) or at the moment of AP induction (treatment). Mucosal barrier function of the distal ileum was assessed in Ussing chambers by measurement of the epithelial electrical resistance and the permeability to Na-fluorescein. RESULTS: Histological analysis revealed pancreatic injury in both phases of AP, and lung damage in the early phase. Epithelial resistance of the ileum was reduced and permeability increased in both phases of AP, indicating impairment of the intestinal barrier. Pretreatment had no effect on resistance or permeability in the early phase of AP. In the late phase of AP, pretreatment but not treatment abolished the AP induced resistance decrease and permeability increase. Administration of probiotics as such (ie, without induction of AP) had no effect on intestinal barrier function. CONCLUSION: Pretreatment with multispecies probiotics for 2 days abolishes intestinal barrier dysfunction in the late phase of AP, while treatment does not. The effectiveness of probiotics in this model depends on the timing of administration. Clinical trials with probiotics should seek conditions where treatment can be started prior to onset of disease or elective surgical intervention.
Authors:
Jakub W Rychter; L Paul van Minnen; André Verheem; Harro M Timmerman; Ger T Rijkers; Marguerite E I Schipper; Hein G Gooszen; Louis M A Akkermans; Alfons B A Kroese
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Surgery     Volume:  145     ISSN:  1532-7361     ISO Abbreviation:  Surgery     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-26     Completed Date:  2009-02-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0417347     Medline TA:  Surgery     Country:  United States    
Other Details:
Languages:  eng     Pagination:  157-67     Citation Subset:  AIM; IM    
Affiliation:
Gastrointestinal Research Unit, Department of Surgery, University Medical Center Utrecht, Utrecht, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Translocation*
Bifidobacterium
Caerulein
Ileal Diseases / etiology,  prevention & control*
Lactobacillus acidophilus
Lactobacillus casei
Lactococcus lactis
Lung / pathology
Male
Mice
Pancreas / pathology
Pancreatitis / complications*,  pathology
Probiotics / administration & dosage*
Chemical
Reg. No./Substance:
17650-98-5/Caerulein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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