Document Detail


Pretransition and progressive softening of bovine carbonic anhydrase II as probed by single molecule atomic force microscopy.
MedLine Citation:
PMID:  15929995     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To develop a simple method for probing the physical state of surface adsorbed proteins, we adopted the force curve mode of an atomic force microscope (AFM) to extract information on the mechanical properties of surface immobilized bovine carbonic anhydrase II under native conditions and in the course of guanidinium chloride-induced denaturation. A progressive increase in the population of individually softened molecules was probed under mildly to fully denaturing conditions. The use of the approach regime of force curves gave information regarding the height and rigidity of the molecule under compressive stress, whereas use of the retracting regime of the curves gave information about the tensile characteristics of the protein. The results showed that protein molecules at the beginning of the transition region possessed slightly more flattened and significantly more softened conformations compared with that of native molecules, but were still not fully denatured, in agreement with results based on solution studies. Thus the force curve mode of an AFM was shown to be sensitive enough to provide information concerning the different physical states of single molecules of globular proteins.
Authors:
Rehana Afrin; Mohammad T Alam; Atsushi Ikai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Protein science : a publication of the Protein Society     Volume:  14     ISSN:  0961-8368     ISO Abbreviation:  Protein Sci.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-06-02     Completed Date:  2005-09-19     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  9211750     Medline TA:  Protein Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1447-57     Citation Subset:  IM    
Affiliation:
Laboratory of Biodynamics, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuka, Midori-ku, Yokohama, 226-8501, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carbonic Anhydrase II / chemistry*
Cattle
Guanidine / chemistry*
Microscopy, Atomic Force*
Protein Denaturation
Chemical
Reg. No./Substance:
113-00-8/Guanidine; EC 4.2.1.-/Carbonic Anhydrase II
Comments/Corrections

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