Document Detail


Pretaporter, a Drosophila protein serving as a ligand for Draper in the phagocytosis of apoptotic cells.
MedLine Citation:
PMID:  19927123     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Phagocytic removal of cells undergoing apoptosis is necessary for animal development and tissue homeostasis. Draper, a homologue of the Caenorhabditis elegans phagocytosis receptor CED-1, is responsible for the phagocytosis of apoptotic cells in Drosophila, but its ligand presumably present on apoptotic cells remains unknown. An endoplasmic reticulum protein that binds to the extracellular region of Draper was isolated. Loss of this protein, which we name Pretaporter, led to a reduced level of apoptotic cell clearance in embryos, and the overexpression of pretaporter in the mutant flies rescued this defect. Results from genetic analyses suggested that Pretaporter functionally interacts with Draper and the corresponding signal mediators. Pretaporter was exposed at the cell surface after the induction of apoptosis, and cells artificially expressing Pretaporter at their surface became susceptible to Draper-mediated phagocytosis. Finally, the incubation with Pretaporter augmented the tyrosine-phosphorylation of Draper in phagocytic cells. These results collectively suggest that Pretaporter relocates from the endoplasmic reticulum to the cell surface during apoptosis to serve as a ligand for Draper in the phagocytosis of apoptotic cells.
Authors:
Takayuki Kuraishi; Yukiko Nakagawa; Kaz Nagaosa; Yumi Hashimoto; Takashi Ishimoto; Takeshi Moki; Yu Fujita; Hiroshi Nakayama; Naoshi Dohmae; Akiko Shiratsuchi; Naoko Yamamoto; Koichi Ueda; Masamitsu Yamaguchi; Takeshi Awasaki; Yoshinobu Nakanishi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The EMBO journal     Volume:  28     ISSN:  1460-2075     ISO Abbreviation:  EMBO J.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-12-16     Completed Date:  2010-01-14     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  8208664     Medline TA:  EMBO J     Country:  England    
Other Details:
Languages:  eng     Pagination:  3868-78     Citation Subset:  IM    
Affiliation:
Graduate School of Medical Science, Kanazawa University, Kanazawa, Ishikawa, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis*
Cell Membrane / metabolism
Drosophila Proteins / genetics,  metabolism,  physiology*
Drosophila melanogaster / genetics,  metabolism
Endoplasmic Reticulum / metabolism
Hemocytes / metabolism
Ligands
Membrane Proteins / genetics*,  physiology*
Microscopy, Fluorescence / methods
Models, Genetic
Mutation
Phagocytes / metabolism
Phagocytosis*
Protein Structure, Tertiary
Chemical
Reg. No./Substance:
0/Draper protein, Drosophila; 0/Drosophila Proteins; 0/Ligands; 0/Membrane Proteins; 0/pretaporter protein, Drosophila
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