Document Detail


Presynaptic interneuron subtype- and age-dependent modulation of GABAergic synaptic transmission by beta-adrenoceptors in rat insular cortex.
MedLine Citation:
PMID:  20457865     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
beta-Adrenoceptors play a crucial role in the regulation of taste aversion learning in the insular cortex (IC). However, beta-adrenergic effects on inhibitory synaptic transmission mediated by gamma-aminobutyric acid (GABA) remain unknown. To elucidate the mechanisms of beta-adrenergic modulation of inhibitory synaptic transmission, we performed paired whole cell patch-clamp recordings from layer V GABAergic interneurons and pyramidal cells of rat IC aged from postnatal day 17 (PD17) to PD46 and examined the effects of isoproterenol, a beta-adrenoceptor agonist, on unitary inhibitory postsynaptic currents (uIPSCs). Isoproterenol (100 microM) induced facilitating effects on uIPSCs in 33.3% of cell pairs accompanied by decreases in coefficient of variation (CV) of the first uIPSC amplitude and paired-pulse ratio (PPR) of the second to first uIPSC amplitude, whereas 35.9% of pairs showed suppressive effects of isoproterenol on uIPSC amplitude obtained from fast spiking (FS) to pyramidal cell pairs. Facilitatory effects of isoproterenol were frequently observed in FS-pyramidal cell pairs at > or =PD24. On the other hand, isoproterenol suppressed uIPSC amplitude by 52.3 and 39.8% in low-threshold spike (LTS)-pyramidal and late spiking (LS)-pyramidal cell pairs, respectively, with increases in CV and PPR. The isoproterenol-induced suppressive effects were blocked by preapplication of 100 microM propranolol, a beta-adrenoceptor antagonist. There was no significant correlation between age and changes of uIPSCs in LTS-/LS-pyramidal cell pairs. These results suggest the presence of differential mechanisms in presynaptic GABA release and/or postsynaptic GABA(A) receptor-related assemblies among interneuron subtypes. Age- and interneuron subtype-specific beta-adrenergic modulation of IPSCs may contribute to experience-dependent plasticity in the IC.
Authors:
Yuko Koyanagi; Kiyofumi Yamamoto; Yoshiyuki Oi; Noriaki Koshikawa; Masayuki Kobayashi
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-24
Journal Detail:
Title:  Journal of neurophysiology     Volume:  103     ISSN:  1522-1598     ISO Abbreviation:  J. Neurophysiol.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-12     Completed Date:  2010-08-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375404     Medline TA:  J Neurophysiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2876-88     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, Nihon University School of Dentistry, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Action Potentials / drug effects,  physiology
Adrenergic alpha-Agonists / pharmacology
Adrenergic beta-Agonists / pharmacology
Aging / drug effects,  physiology*
Animals
Cerebral Cortex / drug effects,  growth & development,  physiology*
Female
Inhibitory Postsynaptic Potentials / drug effects,  physiology
Interneurons / drug effects,  physiology*
Isoproterenol / pharmacology
Male
Patch-Clamp Techniques
Presynaptic Terminals / drug effects,  physiology*
Propranolol
Pyramidal Cells / drug effects,  growth & development,  physiology
Rats
Rats, Transgenic
Receptors, Adrenergic, beta / metabolism*
Synaptic Transmission / drug effects,  physiology*
gamma-Aminobutyric Acid / metabolism*
Chemical
Reg. No./Substance:
0/Adrenergic alpha-Agonists; 0/Adrenergic beta-Agonists; 0/Receptors, Adrenergic, beta; 525-66-6/Propranolol; 56-12-2/gamma-Aminobutyric Acid; 7683-59-2/Isoproterenol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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