Document Detail


Preston and Park-Sanders protocols adapted for semi-quantitative isolation of thermotolerant Campylobacter from chicken rinse.
MedLine Citation:
PMID:  12381404     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human campylobacteriosis has become the major cause of foodborne gastrointestinal diseases in several European countries. In order to implement effective control measures in the primary production, and as a tool in risk assessment studies, it is necessary to have sensitive and quantitative detection methods.Thus, semi-quantitative detection of thermophilic Campylobacter spp. in 20 naturally contaminated chicken rinse samples was carried out using the two most common standard protocols: Preston and Park-Sanders, as proposed by Nordic Committee on Food Analysis (NMKL) and International Standard Organization (ISO), respectively. For both protocols, the chicken rinse samples were prepared in 500 ml buffered peptone water, as recommended in the ISO protocol no. 6887-2. The results indicated that the Preston protocol was superior to the Park-Sanders protocol in supporting growth of Campylobacter spp. In conclusion, the established semi-quantitative assessment using Preston broth could be useful in monitoring the outcome of control programs or quantitative risk assessments.
Authors:
M H Josefsen; P S Lübeck; B Aalbaek; J Hoorfar
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of food microbiology     Volume:  80     ISSN:  0168-1605     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  2003 Jan 
Date Detail:
Created Date:  2002-10-16     Completed Date:  2003-01-07     Revised Date:  2009-11-03    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  177-83     Citation Subset:  IM    
Affiliation:
Danish Veterinary Institute, 27 Bülowsvej, DK-1790 Copenhagen V, Denmark.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacteriological Techniques
Campylobacter / isolation & purification*,  physiology
Chickens
Colony Count, Microbial
Culture Media*
Food Contamination
Food Microbiology
Hot Temperature*
Meat / microbiology*
Risk Assessment
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Culture Media

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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