Document Detail

Pressure-polishing pipettes for improved patch-clamp recording.
MedLine Citation:
PMID:  19078936     Owner:  NLM     Status:  MEDLINE    
Pressure-polishing is a method for shaping glass pipettes for patch-clamp recording. We first developed this method for fabricating pipettes suitable for recording from small (<3 m) neuronal cell bodies. The basic principal is similar to glass-blowing and combines air pressure and heat to modify the shape of patch pipettes prepared by a conventional micropipette puller. It can be applied to so-called soft (soda lime) and hard (borosilicate) glasses. Generally speaking, pressure polishing can reduce pipette resistance by 25% without decreasing the diameter of the tip opening (Goodman and Lockery, 2000). It can be applied to virtually any type of glass and requires only the addition of a high-pressure valve and fitting to a microforge. This technique is essential for recording from ultrasmall cells (<5 m) and can also improve single-channel recording by minimizing pipette resistance. The blunt shape is also useful for perforated-patch clamp recording since this tip shape results in a larger membrane bleb available for perforation.
Brandon E Johnson; Austin L Brown; Miriam B Goodman
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Publication Detail:
Type:  Journal Article; Video-Audio Media     Date:  2008-10-22
Journal Detail:
Title:  Journal of visualized experiments : JoVE     Volume:  -     ISSN:  1940-087X     ISO Abbreviation:  J Vis Exp     Publication Date:  2008  
Date Detail:
Created Date:  2008-12-16     Completed Date:  2009-02-17     Revised Date:  2013-06-04    
Medline Journal Info:
Nlm Unique ID:  101313252     Medline TA:  J Vis Exp     Country:  United States    
Other Details:
Languages:  eng     Pagination:  -     Citation Subset:  IM    
Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA, USA.
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MeSH Terms
Neurons / physiology
Patch-Clamp Techniques / instrumentation*,  methods
Grant Support

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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