Document Detail


Pressure perturbation calorimetry of helical peptides.
MedLine Citation:
PMID:  16372358     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pressure perturbation calorimetry quantifies the temperature dependence of a solute's thermal expansion coefficient, providing information about solute-solvent interactions. We tested the idea that pressure perturbation calorimetry can provide information about solvent-accessible surface area by studying peptides with different secondary structures. The peptides comprised two host-guest series: one predominately an alpha-helix, the other predominately a polyproline II helix. In aqueous buffer, we find a correlation between the amount of secondary structure as assessed by circular dichroism spectropolarimetry and the pressure perturbation calorimetry data. We conclude that pressure perturbation calorimetry can provide information about the exposure of polar and nonpolar surface area. Data acquired in a buffered urea solution, however, are not as easily interpreted.
Authors:
Devin G Barrett; C Michael Minder; Michelle U Mian; Shelly J Whittington; W John Cooper; Kristin M Fuchs; Ashutosh Tripathy; Marcey L Waters; Trevor P Creamer; Gary J Pielak
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Proteins     Volume:  63     ISSN:  1097-0134     ISO Abbreviation:  Proteins     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-03-16     Completed Date:  2007-01-11     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  8700181     Medline TA:  Proteins     Country:  United States    
Other Details:
Languages:  eng     Pagination:  322-6     Citation Subset:  IM    
Copyright Information:
2005 Wiley-Liss, Inc.
Affiliation:
Department of Chemistry, University of North Carolina, Chapel Hill, NC 27599-3290, USA.
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MeSH Terms
Descriptor/Qualifier:
Calorimetry
Circular Dichroism
Peptides / chemistry*
Pressure
Protein Structure, Secondary
Solutions
Temperature
Urea
Grant Support
ID/Acronym/Agency:
GM072691/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Peptides; 0/Solutions; 57-13-6/Urea

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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