Document Detail


Pressure-overload deinduction of human alpha 2 Na,K-ATPase gene expression in transgenic rats.
MedLine Citation:
PMID:  9040446     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The early and sustained deinduction of alpha 2 Na,K-ATPase gene expression in both cardiac left ventricle and aorta in various pressure-overload rat models and in hypertrophied human heart suggests a common transcriptional pressure response mechanism to pressure overload in both rats and humans. To test this hypothesis, we developed transgenic rat lines expressing the chloramphenicol acetyltransferase reporter gene regulated by the human alpha 2 Na,K-ATPase (-798 to +67) regulatory region, H alpha 2-CAT. Analysis of two homozygous transgenic rat lines revealed (1) parallel tissue-specific regulation of the H alpha 2-CAT transgene and rat alpha 2 Na,K-ATPase gene and (2) parallel load-induced deinduction of both cardiac and vascular (aortic) H alpha 2-CAT transgene and rat alpha 2 Na,K-ATPase gene expression in a 3-day model of induced pressure overload. Cardiac H alpha 2-CAT deinduction was detected at a systolic pressure greater than or equal to 150 mm Hg and correlated with the degree of systolic pressure elevation (r = .82, P < .0001). The data suggest a systolic pressure gradient-dependent coordinate pressure-overload transcriptional response mechanism in the heart and aorta, with one of its target genes being the alpha 2 Na,K-ATPase gene in both humans and rats.
Authors:
N Ruiz-Opazo; X H Xiang; V L Herrera
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Hypertension     Volume:  29     ISSN:  0194-911X     ISO Abbreviation:  Hypertension     Publication Date:  1997 Feb 
Date Detail:
Created Date:  1997-03-13     Completed Date:  1997-03-13     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7906255     Medline TA:  Hypertension     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  606-12     Citation Subset:  IM    
Affiliation:
Section of Molecular Genetics, Whitaker Cardiovascular Institute, Evans Department of Medicine, Boston (Mass) University School of Medicine 02118, USA.
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MeSH Terms
Descriptor/Qualifier:
Angiotensin II / pharmacology
Animals
Animals, Genetically Modified
Aorta / metabolism
Blood Pressure / drug effects
Brain / metabolism
Cells, Cultured
Chloramphenicol O-Acetyltransferase
Down-Regulation / genetics*
Humans
Muscle, Skeletal / metabolism
Myocardium / metabolism
Rats
Rats, Sprague-Dawley
Sodium-Potassium-Exchanging ATPase / genetics*,  metabolism*
Stress, Physiological / genetics*
Grant Support
ID/Acronym/Agency:
HL-48903/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
11128-99-7/Angiotensin II; EC 2.3.1.28/Chloramphenicol O-Acetyltransferase; EC 3.6.3.9/Sodium-Potassium-Exchanging ATPase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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