Document Detail


Pressure induced nucleus DNA fragmentation.
MedLine Citation:
PMID:  21234668     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: The present study was designed to investigate the impact of pressure on nuclear DNA integrity in viable cells of mouse blastocysts.
METHODS: The blastocysts of hybrid F1 females [(C57Bl/10 J × CBA-H);N = 15] aged 2-3 months were exposed into the pressure impulse lasting ~0.021 s and characterized by a positive pressure peak of ~76 mmHg. The nuclear DNA fragmentation index of mouse blastocysts was assessed by TUNEL assay within 60 s after exposure to pressure impulse.
RESULTS: The mean nuclear DNA fragmentation index was significantly higher in the experimental group (83%) than in the control group (19.7%); p < 0.001.
CONCLUSION(S): A low magnitude pressure impulse can induce nuclear DNA fragmentation in mouse blastocysts. The compression and decompression forces appearing during pressure fluctuations are responsible for the observed DNA shearing.
Authors:
Cezary Grygoruk; Piotr Sieczynski; Jacek A Modlinski; Barbara Gajda; Pawel Greda; Izabela Grad; Piotr Pietrewicz; Grzegorz Mrugacz
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Publication Detail:
Type:  Journal Article     Date:  2011-01-14
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  28     ISSN:  1573-7330     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-06-15     Completed Date:  2011-10-17     Revised Date:  2013-07-02    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  363-8     Citation Subset:  IM    
Affiliation:
Center for Reproductive Medicine Bocian, Akademicka 26, 15-267, Bialystok, Poland. cezary.grygoruk@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis
Blastocyst / cytology*
Cell Nucleus / metabolism*
DNA Fragmentation*
Female
In Situ Nick-End Labeling
Male
Mice
Mice, Inbred C57BL
Pregnancy
Pressure*
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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