| Pressure induced nucleus DNA fragmentation. | |
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MedLine Citation:
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PMID: 21234668 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: The present study was designed to investigate the impact of pressure on nuclear DNA integrity in viable cells of mouse blastocysts. METHODS: The blastocysts of hybrid F1 females [(C57Bl/10 J × CBA-H);N = 15] aged 2-3 months were exposed into the pressure impulse lasting ~0.021 s and characterized by a positive pressure peak of ~76 mmHg. The nuclear DNA fragmentation index of mouse blastocysts was assessed by TUNEL assay within 60 s after exposure to pressure impulse. RESULTS: The mean nuclear DNA fragmentation index was significantly higher in the experimental group (83%) than in the control group (19.7%); p < 0.001. CONCLUSION(S): A low magnitude pressure impulse can induce nuclear DNA fragmentation in mouse blastocysts. The compression and decompression forces appearing during pressure fluctuations are responsible for the observed DNA shearing. |
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Authors:
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Cezary Grygoruk; Piotr Sieczynski; Jacek A Modlinski; Barbara Gajda; Pawel Greda; Izabela Grad; Piotr Pietrewicz; Grzegorz Mrugacz |
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Publication Detail:
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Type: Journal Article Date: 2011-01-14 |
Journal Detail:
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Title: Journal of assisted reproduction and genetics Volume: 28 ISSN: 1573-7330 ISO Abbreviation: J. Assist. Reprod. Genet. Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-06-15 Completed Date: 2011-10-17 Revised Date: 2012-09-18 |
Medline Journal Info:
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Nlm Unique ID: 9206495 Medline TA: J Assist Reprod Genet Country: Netherlands |
Other Details:
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Languages: eng Pagination: 363-8 Citation Subset: IM |
Affiliation:
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Center for Reproductive Medicine Bocian, Akademicka 26, 15-267, Bialystok, Poland. cezary.grygoruk@gmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis Blastocyst / cytology* Cell Nucleus / metabolism* DNA Fragmentation* Female In Situ Nick-End Labeling Male Mice Mice, Inbred C57BL Pregnancy Pressure* |
| Comments/Corrections | |
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