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Pressure-induced depolymerization of spindle microtubules. III. Differential stability in HeLa cells.
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MedLine Citation:
PMID:  1262399     Owner:  NLM     Status:  MEDLINE    
Evidence from light microscopy (principally polarization microscopy) has demonstrated that hydrostatic pressure can reversibly inhibit mitosis by rapidly depolymerizing the spindle fiber microtubules. We have confirmed this finding in ultrastructural studies of mitotic HeLa cells incubated at 37 degrees C and pressurized at 680 atm (10,000 psi). Althouth there are many spindle microtubules in the cells at atmospheric pressure, electron micographs of cells pressurized for 10 min (and fixed while under pressure in a Landau-Thibodeau chamber) show few microtubules. Pressure has a differential effect on the various types of spindle microtubules. Astral and interpolar MTs appear to be completely depolymerized in pressurized cells, but occasional groups of kinetochore fiber microtubules are seen. Surprisingly, the length and density of microtubules of the stem bodies and midbody of telophase cells appear unchanged by pressurization. In cells fixed 10 min after pressure was released, microtubules were again abundant, the density often appearing to be higher than in control cells. Reorganization seems incomplete, however, since many of the microtubules are randomly oriented. Unexpectedly, kinetochores appeared diffuse and were difficult to identify in sections of pressurized cells. Even after 10 min of recovery at atmospheric pressure, their structure was less distinct than in unpressurized cells.
E D Salmon; D Goode; T K Maugel; D B Bonar
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of cell biology     Volume:  69     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1976 May 
Date Detail:
Created Date:  1976-07-06     Completed Date:  1976-07-06     Revised Date:  2010-09-07    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  443-54     Citation Subset:  IM    
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MeSH Terms
Chromosomes / ultrastructure
Hela Cells
Hydrostatic Pressure*
Microtubules / ultrastructure*
Organoids / ultrastructure

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 5 Year: 1976
Volume: 69 Issue: 2
First Page: 443 Last Page: 454
ID: 2109687
Publisher Id: 76167731
PubMed Id: 1262399

Pressure-induced depolymerization of spindle microtubules. III. Differential stability in HeLa cells

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