Document Detail


Pressure inactivation of Tulane virus, a candidate surrogate for human norovirus and its potential application in food industry.
MedLine Citation:
PMID:  23353553     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Human norovirus (HuNoV) is the leading causative agent for foodborne disease. Currently, studies of HuNoV usually rely on surrogates such as murine norovirus (MNV) due to the lack of a suitable cell culture system and a small animal model for HuNoV. Tulane virus (TV), a monkey calicivirus, is a cultivable enteric calicivirus that not only recognizes the same receptors as HuNoV, but is also genetically closely related to HuNoV. In this study, we determined the pH stability of TV and MNV-1, as well as the effect of high hydrostatic pressure (HHP) on inactivating both viruses in aqueous media, blueberries and oysters. We demonstrated that both TV and MNV-1 were very stable under an acidic environment. They were more resistant to pressure at an acidic environment than at neutral pH. Pressure treatment of 600MPa for 2min at different temperatures (4, 21 and 35°C) barely caused any reduction of TV, as well as MNV-1, on un-wetted (dry) blueberries. However, both TV and MNV-1 on blueberries were successfully inactivated by a pressure of ≤400MPa when blueberries were immersed in phosphate-buffered saline during HHP. Pressure inactivation of both TV and MNV-1 in blueberries and oysters increased as sample temperature decreased in the order of 4>21>35°C. TV was more sensitive to pressure than MNV-1 for the three matrices tested, culture media, blueberries and oysters. This study provides important information on the use of TV as a surrogate for HuNoV study. Results obtained from this study lay a foundation for designing effective HHP treatments for inactivation of HuNoV in high-risk foods such as berries and oysters.
Authors:
Xinhui Li; Mu Ye; Hudaa Neetoo; Serguei Golovan; Haiqiang Chen
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-2
Journal Detail:
Title:  International journal of food microbiology     Volume:  162     ISSN:  1879-3460     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  37-42     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
Affiliation:
Department of Animal and Food Sciences, University of Delaware, Newark, DE 19716-2150, USA.
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