Document Detail


Pressure assisted stabilization of biocatalysts at elevated temperatures: Characterization by dynamic light scattering.
MedLine Citation:
PMID:  23280599     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The effect of pressure, at elevated temperatures, is reported on the activity and stability of a thermophilic endo-ß-Glucanase from the filamentous fungus Talaromyces emersonii. The production of reduced sugars after treatment at different temperatures and pressures is used as a measure of the activity and stability of the enzyme. The activity of the enzyme is maintained to higher temperatures with increasing pressure. For example, the relative activity of endo-ß-Glucanase decreases to 30% after four hours at 75 °C and 1 bar, whereas it is preserved at 100% after six hours at 75 °C and 230 bar. High-pressure dynamic light scattering is used to characterize the hydrodynamic radius of the enzyme as a function of pressure, temperature and time. At higher temperature the hydrodynamic radius increases with time, whereas increasing pressure suppresses this effect. Changes in the hydrodynamic radius are correlated with the activity measurements obtained at elevated pressures, since the changes in the hydrodynamic radius indicate structural changes of the enzyme, which cause the deactivation. Biotechnol. Bioeng. © 2012 Wiley Periodicals, Inc.
Authors:
Christian Kirsch; Jessica Dahms; Andrei F Kostko; Mark A McHugh; Irina Smirnova
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-27
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  -     ISSN:  1097-0290     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2013-1-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Affiliation:
Institute of Thermal Separation Processes, Technical University Hamburg-Harburg, Germany, 21073 Hamburg.
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