Document Detail


Pressure-aided transfusion of platelets: does it affect the platelets?
MedLine Citation:
PMID:  19788509     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: In massively bleeding patients, pressure infusers are used for transfusion of red blood cells and plasma but not for platelets (PLTs) due to an assumed negative effect on the PLTs. This study examined whether pressure-aided in vitro transfusion affected the number, activation state, and/or function of the PLTs as measured by flow cytometry and thrombelastography (TEG). STUDY DESIGN AND METHODS: PLT concentrates stored for 1 (n = 8) or 8 (n = 7) days were transfused in vitro by means of a pressure inducer (300 mmHg). Samples before and after transfusion were measured for PLT concentration and expression of CD62P, CD63, and PAC-1. These activation markers were measured by flow cytometry on resting PLTs as well as PLTs stimulated with thrombin receptor-activating peptide. Clot generation and strength was examined by TEG by measuring the angle (degree) and maximum amplitude (mm), values that are highly dependent on the PLT function. RESULTS: PLT concentrations were unchanged after pressure-aided transfusion reflecting no destruction. With respect to activation state and in vitro functional capacity either no or only minor differences (<7%) were detected. CONCLUSION: In this study, use of a pressure inducer decreased the transfusion time of in vitro PLT transfusion by approximately 50%. No or only minor and inconsistent changes of the PLT number and function were observed. Consequently, this study indicates that pressure infusers could be used for transfusion of PLTs if clinically indicated, that is, in massively bleeding patients. However, in vivo studies to assess the safety and utility of pressure-aided PLT transfusion are warranted.
Authors:
Anne Fischer-Nielsen; Trine Stissing; Charlotte Maansson; Bent Gymoese Joergensen
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-09-24
Journal Detail:
Title:  Transfusion     Volume:  50     ISSN:  1537-2995     ISO Abbreviation:  Transfusion     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-03-17     Completed Date:  2010-03-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0417360     Medline TA:  Transfusion     Country:  United States    
Other Details:
Languages:  eng     Pagination:  361-5     Citation Subset:  IM    
Affiliation:
Department of Clinical Immunology, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark. anne.fischer-nielsen@rh.regionh.dk
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MeSH Terms
Descriptor/Qualifier:
Blood Platelets / cytology*
Catheterization
Flow Cytometry
Humans
Platelet Activation
Platelet Count
Platelet Transfusion / instrumentation,  methods*
Pressure*
Stress, Mechanical
Time Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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