Document Detail


Prespore cell fate bias in G1 phase of the cell cycle in Dictyostelium discoideum.
MedLine Citation:
PMID:  16215182     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
By generating a population of Dictyostelium cells that are in the G1 phase of the cell cycle we have examined the influence of cell cycle status on cell fate specification, cell type proportioning and its regulation, and terminal differentiation. The lack of observable mitosis during the development of these cells and the quantification of their cellular DNA content suggests that they remain in G1 throughout development. Furthermore, chromosomal DNA synthesis was not detectable these cells, indicating that no synthesis phase had occurred, although substantial mitochondrial DNA synthesis did occur in prespore cells. The G1-phase cells underwent normal morphological development and sporulation but displayed an elevated prespore/prestalk ratio of 5.7 compared to the 3.0 (or 3:1) ratio normally observed in populations dominated by G2-phase cells. When migrating slugs produced by G1-phase cells were bisected, each half could reestablish the 5.7 (or 5.7:1) prespore/prestalk ratio. These results demonstrate that Dictyostelium cells can carry out the entire developmental cycle in the G1 phase of the cell cycle and that passage from G2 into G1 phase is not required for sporulation. Our results also suggest that the population asymmetry provided by the distribution of cells around the cell cycle at the time of starvation is not strictly required for cell type proportioning. Finally, when developed together with G2-phase cells, G1-phase cells preferentially become prespore cells and exclude G2-phase cells from the prespore-spore cell population, suggesting that G1-phase cells have an advantage over G2-phase cells in executing the spore cell differentiation pathway.
Authors:
Guokai Chen; Adam Kuspa
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Eukaryotic cell     Volume:  4     ISSN:  1535-9778     ISO Abbreviation:  Eukaryotic Cell     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-10-10     Completed Date:  2005-12-21     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  101130731     Medline TA:  Eukaryot Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1755-64     Citation Subset:  IM    
Affiliation:
Verna and Mars McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation*
Culture Media / chemistry
DNA, Mitochondrial / metabolism
DNA, Protozoan / biosynthesis,  metabolism
Dictyostelium / cytology*,  genetics,  growth & development*
G1 Phase / physiology*
G2 Phase / physiology*
Protozoan Proteins / genetics,  metabolism
Recombinant Fusion Proteins / genetics,  metabolism
Spores, Protozoan / metabolism*
Grant Support
ID/Acronym/Agency:
GM52359/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media; 0/DNA, Mitochondrial; 0/DNA, Protozoan; 0/Protozoan Proteins; 0/Recombinant Fusion Proteins; 107565-44-6/COTB protein, Dictyostelium discoideum
Comments/Corrections

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