Document Detail

Preserved and unpreserved 12 anti-allergic ophthalmic solutions and ocular surface toxicity: in vitro assessment in four cultured corneal and conjunctival epithelial cell lines.
MedLine Citation:
PMID:  21212507     Owner:  NLM     Status:  MEDLINE    
In the present study, we evaluated the cytotoxicity of anti-allergic ophthalmic solutions in cultured corneal and conjunctival cells, namely SIRC (rabbit corneal epithelium), BCE C/D-1b (bovine corneal epithelial cells), RC-1 (rabbit corneal epithelium), and Chang (human conjunctival cells). The viability of cell cultures was determined following the exposure of cells to 12 commercially available anti-allergic ophthalmic solutions for varying exposure times and at various dilutions using the MTT and neutral red assays. The cell viability score (CVS) was used to compare the toxicity of different drugs. Based on CVS data, the order of cell viability after exposure to the drugs was Zepelin ≥ Tramelas PF ≥ Cumorol PF ≥ Ketotifen PF ≥ Eyevinal = Fumarton ≥ Cumorol > Intal ≥ Rizaben ≥ Tramelas ≥ Patanol Livostin. In conclusion, cell viability was mostly affected by the concentration of benzalkonium chloride rather than the active component and/or the anti-allergic action of the drug. The CVS was useful in comparing the toxicity of different drugs.
Masahiko Ayaki; Atsuo Iwasawa; Shigeo Yaguchi; Ryohei Koide
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biocontrol science     Volume:  15     ISSN:  1342-4815     ISO Abbreviation:  Biocontrol Sci     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2011-01-07     Completed Date:  2011-01-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9712121     Medline TA:  Biocontrol Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  143-8     Citation Subset:  IM    
Department of Ophthalmology, Saitama National Hospital.
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MeSH Terms
Anti-Allergic Agents / toxicity*
Cell Line
Cell Survival / drug effects
Conjunctiva / cytology,  drug effects*
Cornea / cytology,  drug effects*
Epithelium / drug effects
Ophthalmic Solutions / toxicity*
Preservatives, Pharmaceutical
Risk Assessment
Reg. No./Substance:
0/Anti-Allergic Agents; 0/Ophthalmic Solutions; 0/Preservatives, Pharmaceutical

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