| Preserved and unpreserved 12 anti-allergic ophthalmic solutions and ocular surface toxicity: in vitro assessment in four cultured corneal and conjunctival epithelial cell lines. | |
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MedLine Citation:
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PMID: 21212507 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the present study, we evaluated the cytotoxicity of anti-allergic ophthalmic solutions in cultured corneal and conjunctival cells, namely SIRC (rabbit corneal epithelium), BCE C/D-1b (bovine corneal epithelial cells), RC-1 (rabbit corneal epithelium), and Chang (human conjunctival cells). The viability of cell cultures was determined following the exposure of cells to 12 commercially available anti-allergic ophthalmic solutions for varying exposure times and at various dilutions using the MTT and neutral red assays. The cell viability score (CVS) was used to compare the toxicity of different drugs. Based on CVS data, the order of cell viability after exposure to the drugs was Zepelin ≥ Tramelas PF ≥ Cumorol PF ≥ Ketotifen PF ≥ Eyevinal = Fumarton ≥ Cumorol > Intal ≥ Rizaben ≥ Tramelas ≥ Patanol Livostin. In conclusion, cell viability was mostly affected by the concentration of benzalkonium chloride rather than the active component and/or the anti-allergic action of the drug. The CVS was useful in comparing the toxicity of different drugs. |
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Authors:
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Masahiko Ayaki; Atsuo Iwasawa; Shigeo Yaguchi; Ryohei Koide |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biocontrol science Volume: 15 ISSN: 1342-4815 ISO Abbreviation: Biocontrol Sci Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2011-01-07 Completed Date: 2011-01-25 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9712121 Medline TA: Biocontrol Sci Country: Japan |
Other Details:
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Languages: eng Pagination: 143-8 Citation Subset: IM |
Affiliation:
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Department of Ophthalmology, Saitama National Hospital. mayaki@olive.ocn.ne.jp |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anti-Allergic Agents / toxicity* Cattle Cell Line Cell Survival / drug effects Conjunctiva / cytology, drug effects* Cornea / cytology, drug effects* Epithelium / drug effects Humans Ophthalmic Solutions / toxicity* Preservatives, Pharmaceutical Rabbits Risk Assessment |
| Chemical | |
Reg. No./Substance:
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0/Anti-Allergic Agents; 0/Ophthalmic Solutions; 0/Preservatives, Pharmaceutical |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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