Document Detail


Preserved endothelial integrity and nitric oxide synthase in saphenous vein grafts harvested by a 'no-touch' technique.
MedLine Citation:
PMID:  11531869     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The saphenous vein is the most commonly used conduit for coronary artery bypass surgery, but 1-year occlusion rates as high as 30 per cent have been reported. In conventional surgery, considerable damage to the vein occurs during harvesting. The aim of this study was to compare endothelial integrity and nitric oxide synthase (NOS) in saphenous veins harvested by a novel 'no-touch' technique and veins harvested conventionally. METHODS: Immunohistochemistry was used to study endothelial integrity, and a combination of histochemistry and autoradiography was employed to identify NOS in human saphenous veins harvested by conventional and no-touch techniques. RESULTS: The endothelial lining of conventional grafts was reduced compared with that of no-touch grafts (52 versus 73 per cent; P = 0.04). This was associated with a concomitant reduction of NOS availability; NOS was also present in the adventitial vasa vasorum of no-touch vessels. CONCLUSION: Some of the sites with potential for nitric oxide release in vivo are removed during conventional saphenous vein harvesting. These sites were preserved after no-touch harvesting, suggesting the potential to improve coronary artery bypass graft patency.
Authors:
J C Tsui; D S Souza; D Filbey; V Bomfim; M R Dashwood
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  The British journal of surgery     Volume:  88     ISSN:  0007-1323     ISO Abbreviation:  Br J Surg     Publication Date:  2001 Sep 
Date Detail:
Created Date:  2001-09-04     Completed Date:  2001-09-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372553     Medline TA:  Br J Surg     Country:  England    
Other Details:
Languages:  eng     Pagination:  1209-15     Citation Subset:  AIM; IM    
Affiliation:
Department of Surgery, Royal Free and University College Medical School, London, UK.
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MeSH Terms
Descriptor/Qualifier:
Aged
Aged, 80 and over
Autoradiography
Collagen / metabolism
Coronary Artery Bypass / methods
Endothelium, Vascular / enzymology*,  pathology
Humans
Immunohistochemistry
Middle Aged
NADPH Dehydrogenase / metabolism
Nitric Oxide Synthase / metabolism*
Saphenous Vein / enzymology,  pathology,  transplantation*
Specimen Handling / adverse effects
Chemical
Reg. No./Substance:
9007-34-5/Collagen; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.6.99.1/NADPH Dehydrogenase

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