| Preservation of transendothelial glucose transporter 1 and P-glycoprotein transporters in a cortical slice culture model of the blood-brain barrier. | |
| | |
MedLine Citation:
|
PMID: 20603190 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
A variety of neurological diseases are characterized by disturbances of the blood-brain barrier (BBB) and its transporters. We recently introduced fibroblast growth factor treated cortical organotypic slice cultures of mice as a model for in vitro studies of the blood-brain barrier and have now further characterized the maintenance and function of transport-proteins typically expressed in the endothelium of cerebral blood vessels. The glucose transporter GLUT-1 is present in blood vessels of slice cultures derived from postnatal day 4 to 21 mice after 3 days in vitro. The endothelial multidrug resistance P-glycoprotein (P-gp) which is involved in the control of pharmacological substance transport across the blood-brain barrier is also maintained in blood vessels, most prominently in slice cultures derived from postnatal day 14 and 21 mice. To assess P-gp function, we tested rhodamine 123 transport in presence or absence of the P-gp inhibitor verapamil. Rhodamine 123-fluorescence accumulated rapidly in the vascular lumen both in acute slices and in slices cultured for 3 days in vitro. Our results provide evidence that endothelial transporters and their functional properties can be maintained in organotypic cortical slices cultures, thus making it an attractive model system for the study of the blood-brain barrier. |
| | |
Authors:
|
R S Camenzind; S Chip; H Gutmann; J P Kapfhammer; C Nitsch; K Bendfeldt |
Publication Detail:
|
Type: Comparative Study; Journal Article Date: 2010-07-09 |
Journal Detail:
|
Title: Neuroscience Volume: 170 ISSN: 1873-7544 ISO Abbreviation: Neuroscience Publication Date: 2010 Sep |
Date Detail:
|
Created Date: 2010-08-23 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7605074 Medline TA: Neuroscience Country: United States |
Other Details:
|
Languages: eng Pagination: 361-71 Citation Subset: IM |
Copyright Information:
|
Copyright 2010 IBRO. Published by Elsevier Ltd. All rights reserved. |
Affiliation:
|
Section of Functional Neuroanatomy, Institute of Anatomy, Department of Biomedicine, University of Basel, Pestalozzistrasse 20, CH-4056 Basel, Switzerland. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Person perception precedes theory of mind: an event related potential analysis.
Next Document: Olfactory type G-protein alpha subunit in striosome-matrix dopamine systems in adult mice.