| Preservation of steatotic livers: a comparison between cold storage and machine perfusion preservation. | |
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MedLine Citation:
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PMID: 17394146 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Liver grafts are frequently discarded due to steatosis. Steatotic livers can be classified as suboptimal and deteriorate rapidly during hypothermic static preservation, often resulting in graft nonfunction. Hypothermic machine perfusion (MP) has been introduced for preservation of donor livers instead of cold storage (CS), resulting in superior preservation outcomes. The aim of this study was to compare CS and MP for preservation of the steatotic donor rat liver. Liver steatosis was induced in male Wistar rats by a choline-methionine-deficient diet. After 24 hours hypothermic CS using the University of Wisconsin solution (UW) or MP using UW-Gluconate (UW-G), liver damage (liver enzymes, perfusate flow, and hyaluronic acid clearance) and liver function (bile production, ammonia clearance, urea production, oxygen consumption, adenosine triphosphate [ATP] levels) were assessed in an isolated perfused rat liver model. Furthermore, liver biopsies were visualized by hematoxylin and eosin staining. Animals developed 30 to 60% steatosis. Livers preserved by CS sustained significantly more damage as compared to MP. Bile production, ammonia clearance, urea production, oxygen consumption, and ATP levels were significantly higher after MP as compared to CS. These results were confirmed by histology. In conclusion, MP improves preservation results of the steatotic rat liver, as compared to CS. |
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Authors:
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Maud Bessems; Benedict M Doorschodt; Joe L P Kolkert; Reeta L Vetelainen; Arlene K van Vliet; Heleen Vreeling; Jan van Marle; Thomas M van Gulik |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society Volume: 13 ISSN: 1527-6465 ISO Abbreviation: Liver Transpl. Publication Date: 2007 Apr |
Date Detail:
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Created Date: 2007-04-16 Completed Date: 2007-06-13 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 100909185 Medline TA: Liver Transpl Country: United States |
Other Details:
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Languages: eng Pagination: 497-504 Citation Subset: IM |
Affiliation:
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Surgical Laboratory, Department of Surgery, Academic Medical Center, Amsterdam, The Netherlands. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cold Temperature Fatty Liver* / pathology Humans Liver Function Tests Liver Transplantation / physiology* Male Methionine / deficiency Models, Animal Organ Preservation / methods* Perfusion Rats Rats, Wistar Reperfusion / methods Tissue Donors* |
| Chemical | |
Reg. No./Substance:
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63-68-3/Methionine |
| Comments/Corrections | |
Comment In:
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Liver Transpl. 2007 Apr;13(4):480-2
[PMID:
17394143
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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