Document Detail


Preservation of sarcoplasmic reticulum Ca2(+)-sequestering function in homogenates of different type composition following sprint activity.
MedLine Citation:
PMID:  7882189     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To examine the effect of exercise on sarcoplasmic reticulum function in muscle tissue of different fibre composition, adult male Wistar rats weighing 388 +/- 23 g (x +/- SE) ran intermittently on a treadmill until fatigue. Fatigue was induced by 15-20 min of running performed at 52 m/min on an 8 degrees incline in periods of 2.5 min of exercise separated by 2 min of recovery. Analysis of sarcoplasmic reticulum Ca2+ ATPase activity determined in homogenates indicated no difference (p > 0.05) between age-matched control and exercised tissue for the soleus (SOL; 0.121 +/- 0.012 vs. 0.156 +/- 0.018 mumol.mg-1 protein.min-1), red gastrocnemius (RG; 0.381 +/- 0.022 vs. 0.354 +/- 0.022), or white gastrocnemius (WG; 0.526 +/- 0.05 vs. 0.471 +/- 0.031). Similarly, both total ATPase and Mg2+ ATPase activities were unaffected by the exercise in any of the tissues examined. Exercise also failed to alter sarcoplasmic reticulum Ca2+ uptake in homogenates of the SOL (1.43 +/- 0.15 vs. 1.38 +/- 0.19 nmol.mg-1 protein.min-1), RG (3.74 +/- 0.29 vs. 3.59 +/- 0.24), and WG (5.98 +/- 0.48 vs. 5.41 +/- 0.50). At fatigue, glycogen depletion was similar in all tissue types and amounted to 65.1% in the SOL (172 +/- 9 vs. 60 +/- 16 mmol.glucosyl units-1.kg-1 dry weight), 74.4% in RG (164 +/- 8 vs. 42 +/- 6), and 79% in the WG (167 +/- 9 vs. 35 +/- 9). It is concluded that exercise by itself does not alter sarcoplasmic reticulum Ca2(+)-sequestering function in tissues of primarily different fibre composition when determined in homogenates in vitro.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
J Dossett-Mercer; H J Green; E Chin; F Grange
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Canadian journal of physiology and pharmacology     Volume:  72     ISSN:  0008-4212     ISO Abbreviation:  Can. J. Physiol. Pharmacol.     Publication Date:  1994 Oct 
Date Detail:
Created Date:  1995-04-12     Completed Date:  1995-04-12     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0372712     Medline TA:  Can J Physiol Pharmacol     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  1231-7     Citation Subset:  IM; S    
Affiliation:
Department of Kinesiology, University of Waterloo, ON, Canada.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphatases / analysis
Animals
Calcium / metabolism*
Calcium-Transporting ATPases / analysis*
Creatine / analysis
Glycogen / analysis
Lactates / analysis
Lactic Acid
Male
Muscle, Skeletal / metabolism
Nucleotides / analysis
Phosphates / analysis
Physical Exertion / physiology
Pyruvates / analysis
Pyruvic Acid
Rats
Rats, Wistar
Sarcoplasmic Reticulum / metabolism*
Chemical
Reg. No./Substance:
0/Lactates; 0/Nucleotides; 0/Phosphates; 0/Pyruvates; 127-17-3/Pyruvic Acid; 50-21-5/Lactic Acid; 57-00-1/Creatine; 7440-70-2/Calcium; 9005-79-2/Glycogen; EC 3.6.1.-/Adenosine Triphosphatases; EC 3.6.1.8/Calcium-Transporting ATPases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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