Document Detail

Preservation of membrane phospholipids by propranolol, pindolol, and metoprolol: a novel mechanism of action of beta-blockers.
MedLine Citation:
PMID:  1684206     Owner:  NLM     Status:  MEDLINE    
In this study, we examined the effects of three different beta-blockers, propranolol, pindolol, and metoprolol, on membrane phospholipid preservation in the ischemic and reperfused rat heart. Isolated rat hearts were perfused with Krebs-Henseleit bicarbonate buffer by the Langdendorff technique in the presence or absence of propranolol, pindolol, or metroprolol (20 microM each) for 15 mins at 37 degrees C. Hearts where then either made ischemic alone at 37 degrees C for 30 mins, or followed by 30 mins of reperfusion. Coronary flow and perfusate creatine kinase content were monitored during both pre- and post-ischemic periods. At the end of the experiment, hearts were frozen by freeze-clamping at liquid nitrogen temperature. Membrane phospholipids, fatty acid composition of these phospholipids, non-esterified free fatty acids, and myocardial thiobabituric acid (TBA) reactive product were examined in these hearts. The beta-blocker-treated hearts exhibited significantly less lipid peroxidation than the control hearts (P less than 0.05), as indicated by decreased formation of TBA reactive product and the higher percentage of unsaturated fatty acids in the phosphatidylcholine (PC) in heart. In addition, compared to the control group, less accumulation of free fatty acids was observed in the propranolol and pindolol treated groups. Finally, reduced myocardial creatine kinase release and enhanced recovery of coronary flow indicated significant myocardial preservation by these beta-blockers. The efficacy of these beta-blockers were in the following order: propranolol, pindolol, metoprolol. These results suggest that beta-blockers could also protect an ischemic heart from reperfusion injury by preserving the membrane phospholipids.
X K Liu; R M Engelman; H R Agrawal; D K Das
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of molecular and cellular cardiology     Volume:  23     ISSN:  0022-2828     ISO Abbreviation:  J. Mol. Cell. Cardiol.     Publication Date:  1991 Oct 
Date Detail:
Created Date:  1992-01-21     Completed Date:  1992-01-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0262322     Medline TA:  J Mol Cell Cardiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1091-100     Citation Subset:  IM    
Department of Surgery, University of Connecticut School of Medicine, Farmington 06010.
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MeSH Terms
Adrenergic beta-Antagonists / pharmacology*
Coronary Circulation / drug effects,  physiology
Coronary Disease / metabolism*
Creatine Kinase / metabolism
Fatty Acids / metabolism
Heart Rate / drug effects,  physiology
Lipid Peroxidation / drug effects
Membrane Lipids / metabolism*
Metoprolol / pharmacology
Myocardial Reperfusion
Myocardium / metabolism*
Phosphatidylcholines / metabolism
Phospholipids / metabolism*
Pindolol / pharmacology*
Propranolol / pharmacology
Rats, Inbred Strains
Reperfusion Injury / prevention & control*
Grant Support
Reg. No./Substance:
0/Adrenergic beta-Antagonists; 0/Fatty Acids; 0/Membrane Lipids; 0/Phosphatidylcholines; 0/Phospholipids; 13523-86-9/Pindolol; 37350-58-6/Metoprolol; 525-66-6/Propranolol; EC Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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