Document Detail

Preservation of continuous cell lines at -85°C: a low-cost alternative for resource limited countries.
MedLine Citation:
PMID:  21045405     Owner:  NLM     Status:  In-Process    
BACKGROUND: Cell culture is the most popular method of virus propagation because of its high sensitivity. However, the need of high cost liquid nitrogen for storage of cell lines is one of the main limiting factor for its widespread use in developing countries.
OBJECTIVE: The present study was therefore carried out to standardize the preservation of continuous cell lines at deep freezer (-85ºC) for 6 months.
METHODS: Fixed number of Vero and Hep2 cells were preserved at -85ºC deep freezer in separate vials and were revived at one month interval to check the growth pattern.
RESULTS: Both the cell lines could be revived with healthy cells and monolayer was formed within 7-10 days, after storage at -85ºC for 4 months.
CONCLUSION: The present study highlights the utility of -85ºC deep freezer as an alternative to liquid N 2 for preservation of these cell lines at least up to four months.
Baijayantimala Mishra; S R Ragini; I L Kashiv; R K Ratho
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Indian journal of pathology & microbiology     Volume:  53     ISSN:  0974-5130     ISO Abbreviation:  Indian J Pathol Microbiol     Publication Date:    2010 Oct-Dec
Date Detail:
Created Date:  2010-11-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7605904     Medline TA:  Indian J Pathol Microbiol     Country:  India    
Other Details:
Languages:  eng     Pagination:  742-4     Citation Subset:  IM    
Department of Virology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
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