| Presentation of the Mls-1 superantigen by human HLA class II molecules to murine T cells. | |
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MedLine Citation:
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PMID: 8395548 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Superantigens (SAG) presented in the context of MHC class II proteins stimulate a strong proliferative response in T cells expressing particular TCR V beta genes. Although this T-cell recognition is not MHC restricted, a strong hierarchy is observed in the ability of various MHC class II molecules to present SAG. Mls-1, encoded by the Murine Mammary Tumor Virus (MMTV) Mtv-7 sag gene, is the prototype of endogenous SAG. In the present study, we have analyzed whether this retroviral gene product can be presented in the context of human HLA class II proteins to murine T cells. Positive results were obtained with the DR isotype and in in vitro, as well as in vivo T-cell stimulation assays. However, the various DR beta alleles, expressed in combination with an identical DR alpha chain, differed in their Mls-1 presenting capacity, indicating that the MHC class II beta-chain contains the primary contact site for Mls-1. Interestingly, the same pattern of TCR V beta restriction was seen in response to Mls-1 presented in the context of human and mouse class II, suggesting that the TCR V beta specificity is uniquely determined by the retroviral SAG. Furthermore, Mls-1 presented in the context of the DQw1 and DPw2 isotypes did not elicit a T-cell response. The results from this study form the basis for further analysis of the exact region in the class II beta-chain that interacts with Mls-1. |
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Authors:
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M Subramanyam; B McLellan; N Labrecque; R P Sekaly; B T Huber |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 151 ISSN: 0022-1767 ISO Abbreviation: J. Immunol. Publication Date: 1993 Sep |
Date Detail:
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Created Date: 1993-09-28 Completed Date: 1993-09-28 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985117R Medline TA: J Immunol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2538-45 Citation Subset: AIM; IM |
Affiliation:
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Department of Pathology, Tufts University School of Medicine, Boston, MA 02111. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alleles Animals Antibodies, Monoclonal / immunology HLA-DR Antigens / genetics, immunology Histocompatibility Antigens Class II / immunology* Lymphocyte Activation Mammary Tumor Virus, Mouse / genetics, immunology* Mice Mice, Inbred CBA Minor Lymphocyte Stimulatory Antigens / immunology* Receptors, Antigen, T-Cell, alpha-beta / analysis, physiology T-Lymphocytes / immunology* |
| Grant Support | |
ID/Acronym/Agency:
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R0I AI-14910/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/HLA-DR Antigens; 0/Histocompatibility Antigens Class II; 0/Minor Lymphocyte Stimulatory Antigens; 0/Receptors, Antigen, T-Cell, alpha-beta |
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