Document Detail


Presensitization of human cells with extrinsic signals to induced chemical carcinogenesis.
MedLine Citation:
PMID:  7216548     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Foreskin-derived low-passage human cell populations were reproducibly transformed with chemical carcinogens when the cells were blocked in G1, released from the block, and treated with either the carcinogen N-methyl-N-nitro-N-nitrosoguanidine (MNNG) or with Aflatoxin B1 in the S period of the cell cycle. Arginine- and glutamine-deficient medium was required to effectively block the cells in the G1 period. Estradiol, insulin, anthralin or phorbol myristate acetate sensitized the cell population to carcinogen treatment when added 10 h before the carcinogen in early S period. Presensitized cells kept blocked in G1 period for 48 h or longer, released and treated in S period with MNNG or Aflatoxin B1 were not transformed; nor did transformation occur in presensitized cell populations treated in G2 (4.5 h), M (1.5 h) or G1 (8.2 h). Cells derived from carcinogen-treated presensitized cells grew as colonies in soft agar at 16-20 PDL. When cells derived from colonies isolated from the soft agar were injected subcutaneously into nude mice, tumors developed.
Authors:
G E Milo; J A DiPaolo
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  International journal of cancer. Journal international du cancer     Volume:  26     ISSN:  0020-7136     ISO Abbreviation:  Int. J. Cancer     Publication Date:  1980 Dec 
Date Detail:
Created Date:  1981-06-13     Completed Date:  1981-06-13     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0042124     Medline TA:  Int J Cancer     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  805-12     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aflatoxins / pharmacology*
Animals
Cell Line
Cell Transformation, Neoplastic / chemically induced*
Cells, Cultured
Culture Media
Humans
Interphase / drug effects
Methylnitronitrosoguanidine / pharmacology*
Mice
Mice, Nude
Neoplasms, Experimental / chemically induced
Skin / cytology
Grant Support
ID/Acronym/Agency:
R0I-CA25907/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Aflatoxins; 0/Culture Media; 70-25-7/Methylnitronitrosoguanidine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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