Document Detail

Presensitization of human cells with extrinsic signals to induced chemical carcinogenesis.
MedLine Citation:
PMID:  7216548     Owner:  NLM     Status:  MEDLINE    
Foreskin-derived low-passage human cell populations were reproducibly transformed with chemical carcinogens when the cells were blocked in G1, released from the block, and treated with either the carcinogen N-methyl-N-nitro-N-nitrosoguanidine (MNNG) or with Aflatoxin B1 in the S period of the cell cycle. Arginine- and glutamine-deficient medium was required to effectively block the cells in the G1 period. Estradiol, insulin, anthralin or phorbol myristate acetate sensitized the cell population to carcinogen treatment when added 10 h before the carcinogen in early S period. Presensitized cells kept blocked in G1 period for 48 h or longer, released and treated in S period with MNNG or Aflatoxin B1 were not transformed; nor did transformation occur in presensitized cell populations treated in G2 (4.5 h), M (1.5 h) or G1 (8.2 h). Cells derived from carcinogen-treated presensitized cells grew as colonies in soft agar at 16-20 PDL. When cells derived from colonies isolated from the soft agar were injected subcutaneously into nude mice, tumors developed.
G E Milo; J A DiPaolo
Related Documents :
2432018 - Characterization of the growth inhibited substate induced in murine hepatic tumor cells...
6304118 - Induction of reverse transformation and normal cell cycle regulation by dibutyryl camp ...
11281638 - A nuclear protein tyrosine phosphatase induces shortening of g1 phase and increase in c...
10527548 - Lovastatin induces mitotic abnormalities in various cell lines.
1881918 - Suppressed intrinsic catalytic activity of glut1 glucose transporters in insulin-sensit...
11063908 - Evidence for the pro-oxidant effect of gamma-glutamyltranspeptidase-related enzyme.
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  International journal of cancer. Journal international du cancer     Volume:  26     ISSN:  0020-7136     ISO Abbreviation:  Int. J. Cancer     Publication Date:  1980 Dec 
Date Detail:
Created Date:  1981-06-13     Completed Date:  1981-06-13     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0042124     Medline TA:  Int J Cancer     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  805-12     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Aflatoxins / pharmacology*
Cell Line
Cell Transformation, Neoplastic / chemically induced*
Cells, Cultured
Culture Media
Interphase / drug effects
Methylnitronitrosoguanidine / pharmacology*
Mice, Nude
Neoplasms, Experimental / chemically induced
Skin / cytology
Grant Support
Reg. No./Substance:
0/Aflatoxins; 0/Culture Media; 70-25-7/Methylnitronitrosoguanidine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  In vitro NK-activity and in vivo resistance to leukemia: studies of beige, beige//nude and wild-type...
Next Document:  Macrophage content and immunogenicity of C57BL/6J and BALB/cByJ methylcholanthrene-induced sarcomas.