Document Detail


Presence of thyrotropin receptor in infant adipocytes.
MedLine Citation:
PMID:  9545014     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In healthy infants, the levels of TSH are known to peak at 50-100 times adult values during the first days of life. In studies of isolated human infant adipocytes, we have earlier shown that bovine TSH (bTSH) has a strong lipolytic effect, accompanied by a blunted response of adipocytes to catecholamines. In this study, we used human recombinant TSH (hTSH), and incubation of adipocytes with hTSH induced a lipolytic response similar to that obtained with the beta-adrenergic receptor agonist isoprenaline in adipocytes isolated from three infants. The lipolytic effect of hTSH was completely blocked by inhibitory TSH receptor (TSHR) antibodies. The TSHR mediates the effects of TSH in the thyroid, and it has been detected in some extrathyroid tissues, but not in isolated human adipocytes or childhood adipose tissue. In this study, we found TSHR RNA in infant and adult adipose tissues and isolated adipocytes with reverse transcriptase-PCR. The sequence of the amplified PCR product agreed with the published sequence. Northern blot hybridization on RNA prepared from infant adipose tissue showed a transcript of the expected size, and the expression of TSHR seemed higher in infant than in adult adipose tissue. In conclusion, this study indicates that TSH plays an active role in the metabolic adaptation after birth.
Authors:
A Janson; H Rawet; L Perbeck; C Marcus
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Pediatric research     Volume:  43     ISSN:  0031-3998     ISO Abbreviation:  Pediatr. Res.     Publication Date:  1998 Apr 
Date Detail:
Created Date:  1998-05-28     Completed Date:  1998-05-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0100714     Medline TA:  Pediatr Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  555-8     Citation Subset:  IM    
Affiliation:
Department of Pediatrics, Huddinge University Hospital, Karolinska Institute, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Adipocytes / chemistry*
Adipose Tissue / growth & development*
Adult
Animals
Blotting, Northern
Cattle
Child
Humans
Infant
Lipolysis
Polymerase Chain Reaction
RNA / analysis
Receptors, Thyrotropin / analysis*,  genetics
Recombinant Proteins / pharmacology
Thyrotropin / pharmacology
Chemical
Reg. No./Substance:
0/Receptors, Thyrotropin; 0/Recombinant Proteins; 63231-63-0/RNA; 9002-71-5/Thyrotropin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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