Document Detail

Presence of three distinct molecular species of Gi protein alpha subunit. Structure of rat cDNAs and human genomic DNAs.
MedLine Citation:
PMID:  2834384     Owner:  NLM     Status:  MEDLINE    
We have cloned a new species of rat Gi alpha (Gi3 alpha) cDNA and genomic DNAs for three distinct human Gi alpha proteins (Gi1 alpha, Gi2 alpha, and Gi3 alpha). Gi3 alpha cDNA codes for a protein of 354 amino acids (Mr 40,522) whose sequence is closely related but distinct from that of the previously isolated rat Gi alpha (Gi2 alpha). By screening the human genomic libraries with the two rat Gi alpha cDNAs as probes, clones encoding human Gi1 alpha, Gi2 alpha, and Gi3 alpha were isolated. The human Gi2 alpha and Gi3 alpha genes are composed of eight coding exons and seven introns and possess a completely identical exon-intron organization. Southern blot analysis indicates that a single copy of each Gi alpha gene is present per haploid human genome.
H Itoh; R Toyama; T Kozasa; T Tsukamoto; M Matsuoka; Y Kaziro
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  263     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1988 May 
Date Detail:
Created Date:  1988-06-09     Completed Date:  1988-06-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  6656-64     Citation Subset:  IM    
Institute of Medical Science, University of Tokyo, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/J03219;  J03220;  J03221;  M19476;  M20586;  M20587;  M20588;  M20589;  M20590;  M20591;  M20592;  M20593;  M20594;  M20595;  M20596;  M20597;  M20598;  M20599;  M20600;  M20601;  M20602;  M20603;  M20604
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MeSH Terms
Amino Acid Sequence
Base Sequence
Cloning, Molecular
DNA / genetics*
DNA Restriction Enzymes
GTP-Binding Proteins / genetics*
Macromolecular Substances
Molecular Sequence Data
Nucleotide Mapping
Sequence Homology, Nucleic Acid
Species Specificity
Reg. No./Substance:
0/Macromolecular Substances; 9007-49-2/DNA; EC 3.1.21.-/DNA Restriction Enzymes; EC 3.6.1.-/GTP-Binding Proteins

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