| Presence of three distinct molecular species of Gi protein alpha subunit. Structure of rat cDNAs and human genomic DNAs. | |
| | |
MedLine Citation:
|
PMID: 2834384 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We have cloned a new species of rat Gi alpha (Gi3 alpha) cDNA and genomic DNAs for three distinct human Gi alpha proteins (Gi1 alpha, Gi2 alpha, and Gi3 alpha). Gi3 alpha cDNA codes for a protein of 354 amino acids (Mr 40,522) whose sequence is closely related but distinct from that of the previously isolated rat Gi alpha (Gi2 alpha). By screening the human genomic libraries with the two rat Gi alpha cDNAs as probes, clones encoding human Gi1 alpha, Gi2 alpha, and Gi3 alpha were isolated. The human Gi2 alpha and Gi3 alpha genes are composed of eight coding exons and seven introns and possess a completely identical exon-intron organization. Southern blot analysis indicates that a single copy of each Gi alpha gene is present per haploid human genome. |
| | |
Authors:
|
H Itoh; R Toyama; T Kozasa; T Tsukamoto; M Matsuoka; Y Kaziro |
Related Documents
:
|
17701394 - Evolutionary analysis of the apa genes in the phaseolus genus: wild and cultivated bean... 23415324 - Characterization of small rnas and their target genes in wheat seedlings using sequenci... 9952444 - Purification of the trehalase gmtre1 from soybean nodules and cloning of its cdna. gmtr... 8253814 - Alternative extracellular and cytoplasmic domains of the integrin alpha 7 subunit are d... 22593224 - Unidirectional evolutionary transitions in fungal mating systems and the role of transp... 12836664 - Integration of genomic datasets to predict protein complexes in yeast. |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The Journal of biological chemistry Volume: 263 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1988 May |
Date Detail:
|
Created Date: 1988-06-09 Completed Date: 1988-06-09 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 6656-64 Citation Subset: IM |
Affiliation:
|
Institute of Medical Science, University of Tokyo, Japan. |
| Data Bank Information | |
Bank Name/Acc. No.:
|
GENBANK/J03219; J03220; J03221; M19476; M20586; M20587; M20588; M20589; M20590; M20591; M20592; M20593; M20594; M20595; M20596; M20597; M20598; M20599; M20600; M20601; M20602; M20603; M20604 |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA / genetics* DNA Restriction Enzymes GTP-Binding Proteins / genetics* Genes* Humans Macromolecular Substances Molecular Sequence Data Nucleotide Mapping Rats Sequence Homology, Nucleic Acid Species Specificity |
| Chemical | |
Reg. No./Substance:
|
0/Macromolecular Substances; 9007-49-2/DNA; EC 3.1.21.-/DNA Restriction Enzymes; EC 3.6.1.-/GTP-Binding Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: H-ras oncogene-transformed human bronchial epithelial cells (TBE-1) secrete a single metalloprotease...
Next Document: Sequence of the phosphothreonyl regulatory site peptide from inactive maize leaf pyruvate, orthophos...