Document Detail


Presence of surfactant lamellar bodies in normal and diseased sinus mucosa.
MedLine Citation:
PMID:  16024936     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Pulmonary surfactant originates from phospholipid lamellar bodies secreted from the type II epithelial cell of the alveolus. In the lower airway, surfactant optimizes surface tension and oxygen exchange, decreases mucus viscosity and aids in mechanical elimination of inhaled pathogens. In addition to the lung, lamellar bodies have been identified in many other cell types throughout the human body. However, no prior studies have identified lamellar bodies in human sinus mucosa. OBJECTIVES: We performed ultrastructural studies to assess whether lamellar bodies are present in the human sinus in a variety of diseased and normal epithelium. METHODS: We biopsied sinus mucosa from 5 subjects, 1 each with allergic fungal sinusitis, eosinophilic mucin rhinosinusitis, cystic fibrosis, frontal sinus mucocele, and cerebrospinal fluid leak (healthy control). Mouse lung served as a positive control. Specimens were prepared using ferrocyanide-reduced osmium tetroxide and thiocarbohydrazide for fixation (R-OTO method) to avoid extraction of phospholipids during dehydration and were viewed with transmission electron microscopy. RESULTS: We identified lamellar bodies in the sinus mucosa of all patients. Additionally, preservation of mouse lung lamellar bodies confirms that the R-OTO method is a valid technique to preserve these structures. CONCLUSIONS: We describe a simpler, faster technique for identification of cellular phospholipid components than those used previously. Definitive identification of these lamellar bodies within ciliated pseudostratified epithelium of the upper airway indicates that surfactant may have a role in sinus function and pathophysiology.
Authors:
Bradford A Woodworth; Nancy Smythe; Samuel S Spicer; Bradley A Schulte; Rodney J Schlosser
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Publication Detail:
Type:  Journal Article     Date:  2005-07-15
Journal Detail:
Title:  ORL; journal for oto-rhino-laryngology and its related specialties     Volume:  67     ISSN:  0301-1569     ISO Abbreviation:  ORL J. Otorhinolaryngol. Relat. Spec.     Publication Date:  2005  
Date Detail:
Created Date:  2005-11-18     Completed Date:  2006-01-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0334721     Medline TA:  ORL J Otorhinolaryngol Relat Spec     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  199-202     Citation Subset:  IM    
Affiliation:
Department of Otolaryngology, Head and Neck Surgery, Medical University of South Carolina, Charleston, SC 29425, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cerebrospinal Fluid Rhinorrhea / pathology
Cystic Fibrosis / pathology
Cytoplasmic Granules / ultrastructure*
Eosinophilia / pathology
Epithelial Cells / ultrastructure
Frontal Sinus / pathology
Humans
Lung / ultrastructure
Mice
Microscopy, Electron, Transmission
Mucins / analysis
Mucocele / pathology
Mycoses / pathology
Paranasal Sinus Diseases / pathology*
Paranasal Sinuses / ultrastructure*
Phospholipids / analysis
Pulmonary Surfactants / analysis*
Respiratory Hypersensitivity / microbiology,  pathology
Respiratory Mucosa / ultrastructure*
Rhinitis / pathology
Sinusitis / microbiology,  pathology
Tissue Fixation / methods
Chemical
Reg. No./Substance:
0/Mucins; 0/Phospholipids; 0/Pulmonary Surfactants

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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