Document Detail


Presence and some characteristics of peroxisomes in immortalized human trophoblast cells.
MedLine Citation:
PMID:  18379038     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Using morphological and biochemical (Western blot and cell fractionation) methods, we investigated whether peroxisomes are present in human extravillous trophoblast cells. Immortalized extravillous trophoblast cells (TCL-1) were incubated in the presence or absence of 0.5 mM clofibric acid for 3 d. In immunofluorescence staining of trophoblast cells with antibodies anti-catalase and anti-acyl-CoA oxidase (marker enzymes of peroxisomes), electron microscopy and immunoelectron microscopy, peroxisomes were detected in the cells. The size and number of peroxisomes in the trophoblast cells were smaller than those in rat liver. The number of peroxisomes was increased by clofibric acid. In Western blot experiment with antibodies anti-peroxisomal enzymes of beta-oxidation system, densitometric analysis revealed approximately two fold increase in staining by clofibric acid. When we performed cell fractionation experiment using catalase as one of the peroxisomal marker enzymes, the highest activity of catalase was found in the light mitochondrial fraction. Specific activity of catalase in the light mitochondrial fraction was significantly increased to about 1.3 times higher than the control value by clofibric acid treatment. Upon Nycodenz density gradient centrifugation, the catalase activity was concentrated in the density fraction around 1.14-1.15. These findings suggest that microperoxisomes, which have a density smaller than those of rat hepatic peroxisomes, do exist in human extravillous trophoblast cells. It may also be possible to proliferate human peroxisomes in limited quantities using peroxisome proliferator of rodents.
Authors:
Fumie Hashimoto; Shigeta Shimooka; Kaori Iwasaki; Asuka Ono; Maiko Kumaoka; Sadaki Yokota; Satoru Takeda; Masaki Okawara; Hidenori Hayashi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biological & pharmaceutical bulletin     Volume:  31     ISSN:  0918-6158     ISO Abbreviation:  Biol. Pharm. Bull.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-04-01     Completed Date:  2008-05-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9311984     Medline TA:  Biol Pharm Bull     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  546-52     Citation Subset:  IM    
Affiliation:
Faculty of Pharmaceutical Sciences, Josai University; Keyakidai, Sakado, Saitama 350-0295, Japan. hasimoto@josai.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Acid Phosphatase / metabolism
Antilipemic Agents / pharmacology
Blotting, Western
Catalase / metabolism
Cell Fractionation
Cell Line
Centrifugation, Density Gradient
Clofibric Acid / pharmacology
Densitometry
Fluorescent Antibody Technique
Humans
Microscopy, Electron
Peroxisomes / enzymology,  metabolism,  ultrastructure*
Trophoblasts / enzymology,  metabolism,  ultrastructure*
Chemical
Reg. No./Substance:
0/Antilipemic Agents; 882-09-7/Clofibric Acid; EC 1.11.1.6/Catalase; EC 3.1.3.2/Acid Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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