| Presence of serotonin1A (5-HT1A) receptor mRNA without binding of [3H]-8-OH-DPAT in peripheral blood mononuclear cells. | |
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MedLine Citation:
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PMID: 7475972 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The authors investigated the presence of serotonin receptor type 1A (5-HT1A) as labeled by the specific ligand 3H-8 hydroxy-2-(di-N-propylamino)tetralin (3H-8-OH-DPAT) in saturation experiments, and the expression of the mRNA encoding them, in human peripheral blood mononuclear cells (PBMC). In situ hybridization experiments were performed as well. The results, showing that the binding of [3H]-8-OH-DPAT to lymphocyte membranes increased linearly up to 100 nM without reaching saturation, may indicate that the 3H-8-OH-DPAT was not specifically labeling the 5-HT1A receptor. By contrast, the expression studies revealed 5-HT1A mRNA in PBMC. These findings suggest that, despite the presence of mRNA, 5-HT1A receptors are not expressed in PBMC, at least in healthy controls. |
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Authors:
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D Marazziti; L Palego; B Dal Canto; A Rotondo; M Pasqualetti; G Gino; A Lucacchini; H Ladinsky; I Nardi; G B Cassano |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Life sciences Volume: 57 ISSN: 0024-3205 ISO Abbreviation: Life Sci. Publication Date: 1995 |
Date Detail:
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Created Date: 1995-12-21 Completed Date: 1995-12-21 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0375521 Medline TA: Life Sci Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 2197-203 Citation Subset: IM |
Affiliation:
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Institute of Psychiatry, Pisa. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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8-Hydroxy-2-(di-n-propylamino)tetralin
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metabolism* Adult Cell Membrane / metabolism Female Humans In Situ Hybridization Leukocytes, Mononuclear / chemistry, metabolism* Male RNA Probes RNA, Antisense RNA, Messenger / analysis Receptors, Serotonin / genetics*, metabolism* Ribonucleases Tritium |
| Chemical | |
Reg. No./Substance:
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0/RNA Probes; 0/RNA, Antisense; 0/RNA, Messenger; 0/Receptors, Serotonin; 10028-17-8/Tritium; 78950-78-4/8-Hydroxy-2-(di-n-propylamino)tetralin; EC 3.1.-/Ribonucleases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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