| Presence of secretory cellular apoptosis susceptibility protein in cerebrospinal fluids of patients with intracerebral hemorrhage caused by stroke and neurotrauma. | |
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MedLine Citation:
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PMID: 20588233 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: The blood-brain barrier (BBB) is a specialized structure that separates blood vessels from the central nervous system (CNS) and restricts the entry of biomolecules and cells into the brain. Matrix metalloproteinase-2 (MMP-2) produced by interferon-gamma-activated microglia (brain macrophages) is essential for disrupting the glia limitans of BBB, which is critical for lymphocytes penetration into brain capillaries in various CNS disorders. The cellular apoptosis susceptibility (CSE1L/CAS) protein has been shown to regulate MMP-2 secretion. METHODS: We examined if CSE1L played a role in regulating the progression of intracerebral brain hemorrhage disorders. RESULTS: CSE1L was detected by immunoblotting in cerebrospinal fluids (CSFs) of patients with intracerebral hemorrhage brain disorders, including stroke and neurotrauma. Interferon-gamma treatment induced CSE1L expression and increased the secretions of CSE1L and MMP-2 by U937 macrophages. Moreover, tranfection of U937 macrophages with siRNA that targeted CSE1L inhibited interferon-gamma-induced CSE1L and MMP-2 secretion by U937 macrophages. The numbers of lymphocytes in CSF were correlated with the levels of CSE1L and MMP-2 in patients' CSF. CONCLUSIONS: Our results suggest that CSE1L plays a role in regulating MMP-2-mediated BBB breakdown and it may be a target for control of BBB permeability in intracerebral brain hemorrhage disorders. |
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Authors:
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Jai-Nien Tung; Tang-Yi Tsao; Shun-Liang Chen; Cheng-Jeng Tai; Shing-Chuan Shen; Ya-Wen Cheng; Ming-Chung Jiang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Neuro endocrinology letters Volume: 31 ISSN: 0172-780X ISO Abbreviation: Neuro Endocrinol. Lett. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-07-23 Completed Date: 2010-10-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8008373 Medline TA: Neuro Endocrinol Lett Country: Sweden |
Other Details:
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Languages: eng Pagination: 390-8 Citation Subset: IM |
Affiliation:
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Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan, Taiwan. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Aged Aged, 80 and over Blood-Brain Barrier / physiology Brain Injuries / cerebrospinal fluid*, complications Cell Line, Tumor Cellular Apoptosis Susceptibility Protein / cerebrospinal fluid*, genetics Cerebral Hemorrhage / cerebrospinal fluid*, complications Humans Interferon-gamma / antagonists & inhibitors, pharmacology Male Matrix Metalloproteinase 2 / secretion Middle Aged RNA, Small Interfering / genetics Stroke / cerebrospinal fluid*, complications Transfection / methods U937 Cells |
| Chemical | |
Reg. No./Substance:
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0/Cellular Apoptosis Susceptibility Protein; 0/RNA, Small Interfering; 82115-62-6/Interferon-gamma; EC 3.4.24.24/Matrix Metalloproteinase 2 |
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