| Presence and regulation of endocrine gland vascular endothelial growth factor/prokineticin-1 and its receptors in ovarian cells. | |
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MedLine Citation:
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PMID: 12915658 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Endocrine gland vascular endothelial growth factor (EG-VEGF) is a novel angiogenic mitogen selective for endothelial cells (EC) in endocrine glands. EG-VEGF is identical to a protein previously cloned and termed prokineticin (PK)-1. The present study examined the expression of EG-VEGF/PK-1 and its receptors in ovarian steroidogenic cells and EC and compared the regulation of EG-VEGF/PK-1 and VEGF expression in SV40 transformed luteinized human granulosa cell line (SVOG). Normal granulosa or SVOG cells expressed EG-VEGF/PK-1 mRNA. Incubation of SVOG cells with forskolin augmented EG-VEGF/PK-1 expression in a dose-dependent manner. Chemical hypoxia induced by CoCl(2) and desferrioxamine mesylate (100 micro M each) markedly reduced EG-VEGF/PK-1. In contrast, hypoxia significantly elevated VEGF mRNA (VEGF165, 189) and protein secretion. Thrombin, like hypoxia, also induced an opposite effect on VEGF and EG-VEGF/PK-1. Whereas EG-VEGF/PK-1 and VEGF were inversely regulated, steroidogenesis and EG-VEGF/PK-1 were positively correlated in SVOG cells. A distinct pattern of ovarian PK receptor (PK-R) expression was observed in which steroidogenic cells predominantly express PK-R1 receptors, whereas corpus luteum-derived EC express high levels of both PK-R1 and PK-R2. Therefore, acting via either PK-R2 or PK-R1, EG-VEGF/PK-1 may have angiogenic as well as nonangiogenic functions in the ovary. |
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Authors:
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Tatiana Kisliouk; Nitzan Levy; Arye Hurwitz; Rina Meidan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: The Journal of clinical endocrinology and metabolism Volume: 88 ISSN: 0021-972X ISO Abbreviation: J. Clin. Endocrinol. Metab. Publication Date: 2003 Aug |
Date Detail:
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Created Date: 2003-08-13 Completed Date: 2003-09-11 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0375362 Medline TA: J Clin Endocrinol Metab Country: United States |
Other Details:
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Languages: eng Pagination: 3700-7 Citation Subset: AIM; IM |
Affiliation:
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Department of Animal Sciences, The Hebrew University of Jerusalem, Rehovot 76100, Israel. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Angiogenesis Inducing Agents
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biosynthesis* Anoxia / metabolism Cell Line Endocrine Glands / metabolism* Female Forskolin / pharmacology Gastrointestinal Hormones / biosynthesis* Granulosa Cells / metabolism Humans Ovary / cytology, metabolism* Progesterone / pharmacology RNA, Messenger / biosynthesis, genetics Receptors, Vascular Endothelial Growth Factor / biosynthesis* Reverse Transcriptase Polymerase Chain Reaction Thrombin / pharmacology Vascular Endothelial Growth Factor, Endocrine-Gland-Derived |
| Chemical | |
Reg. No./Substance:
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0/Angiogenesis Inducing Agents; 0/Gastrointestinal Hormones; 0/PROK1 protein, human; 0/RNA, Messenger; 0/Vascular Endothelial Growth Factor, Endocrine-Gland-Derived; 57-83-0/Progesterone; 66428-89-5/Forskolin; EC 2.7.10.1/Receptors, Vascular Endothelial Growth Factor; EC 3.4.21.5/Thrombin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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