Document Detail


Presence of heterogeneous peroxisomal populations in the rat nervous tissue.
MedLine Citation:
PMID:  9813222     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Peroxisomes were purified from the nervous tissue of 14-day-old rats by means of a Nycodenz gradient. Peroxisomal enzymes exhibited different sedimentation patterns: dihydroxyacetone phosphate acyl-transferase equilibrates at 1.142 g/ml together with the first peak of catalase; palmitoyl-CoA oxidase and D-amino acid oxidase activities are mainly recovered at 1.154 g/ml; the second peak of catalase is found at 1.175 g/ml. Morphological and semi-quantitative analyses of immunogold-labelled peroxisomes reveal profound heterogeneity of the particles. Very small (=0.2 microm diameter), electron dense vesicles containing catalase or thiolase, but devoid of other tested enzymes, are preferentially found in the light region, together with larger ( > 0.2 < 0.3 microm) and less electron dense palmitoyl-CoA oxidase-positive peroxisomes. At intermediate density (1.154 g/ml) peroxisomes of more uniform size (0.25-0.27 microm), containing palmitoyl-CoA oxidase or thiolase with or without catalase are preferentially found. This population extends toward the densest region of the gradient, where very large D-amino acid oxidase-containing peroxisomes are also found. In this region, smaller peroxisomes, often polymorphic, which are catalase- and thiolase-positive and D-amino acid oxidase/palmitoyl-CoA oxidase-negative, are also observed. The possibility that the heterogeneity of neural peroxisomes may reflect both cellular heterogeneity and ongoing peroxisomal biogenesis is discussed.
Authors:
A M Cimini; I Singh; S Farioli-Vecchioli; L Cristiano; M P Cerú
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1425     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1998 Sep 
Date Detail:
Created Date:  1998-11-30     Completed Date:  1998-11-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  13-26     Citation Subset:  IM    
Affiliation:
Department of Basic and Applied Biology, University of L'Aquila, Coppito L'Aquila, Italy.
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MeSH Terms
Descriptor/Qualifier:
Acetyl-CoA C-Acetyltransferase / metabolism
Animals
Brain / enzymology,  ultrastructure*
Catalase / metabolism
Cell Fractionation
Centrifugation, Density Gradient
Immunoblotting
Microbodies / enzymology,  ultrastructure*
Microscopy, Immunoelectron
Oxidoreductases / metabolism
Rats
Chemical
Reg. No./Substance:
EC 1.-/Oxidoreductases; EC 1.11.1.6/Catalase; EC 1.3.3.-/palmitoyl CoA oxidase; EC 2.3.1.9/Acetyl-CoA C-Acetyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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