Document Detail


Presence of glucose, xylose, and glycerol fermenting bacteria in the deep biosphere of the former Homestake gold mine, South Dakota.
MedLine Citation:
PMID:  23919089     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Eight fermentative bacterial strains were isolated from mixed enrichment cultures of a composite soil sample collected at 1.34 km depth from the former Homestake gold mine in Lead, SD, USA. Phylogenetic analysis of their 16S rRNA gene sequences revealed that these isolates were affiliated with the phylum Firmicutes belonging to genera Bacillus and Clostridium. Batch fermentation studies demonstrated that isolates had the ability to ferment glucose, xylose, or glycerol to industrially valuable products such as ethanol and 1,3-propanediol (PDO). Ethanol was detected as the major fermentation end product in glucose-fermenting cultures at pH 10 with yields of 0.205-0.304 g of ethanol/g of glucose. While a xylose-fermenting strain yielded 0.189 g of ethanol/g of xylose and 0.585 g of acetic acid/g of xylose at the end of fermentation. At pH 7, glycerol-fermenting isolates produced PDO (0.323-0.458 g of PDO/g of glycerol) and ethanol (0.284-0.350 g of ethanol/g of glycerol) as major end products while acetic acid and succinic acid were identified as minor by-products in fermentation broths. These results suggest that the deep biosphere of the former Homestake gold mine harbors bacterial strains which could be used in bio-based production of ethanol and PDO.
Authors:
Gurdeep Rastogi; Raghu N Gurram; Aditya Bhalla; Ramon Gonzalez; Kenneth M Bischoff; Stephen R Hughes; Sudhir Kumar; Rajesh K Sani
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Publication Detail:
Type:  Journal Article     Date:  2013-02-15
Journal Detail:
Title:  Frontiers in microbiology     Volume:  4     ISSN:  1664-302X     ISO Abbreviation:  Front Microbiol     Publication Date:  2013  
Date Detail:
Created Date:  2013-08-06     Completed Date:  2013-08-06     Revised Date:  2013-08-27    
Medline Journal Info:
Nlm Unique ID:  101548977     Medline TA:  Front Microbiol     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  18     Citation Subset:  -    
Affiliation:
Department of Chemical and Biological Engineering, South Dakota School of Mines and Technology Rapid City, SD, USA.
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